Effect of Helicobacter pylori infection on NF-κB/NLRP3 signaling pathway and inflammatory factors in patients with gastric cancer
OBJECTIVE To investigate the effects of Helicobacter pylori infection on the NF-κB/NLRP3 signaling pathway and inflammatory factors in patients with gastric cancer.METHODS Totally 49 Hp-positive gastric cancer patients admitted to Tianjin Fifth Central Hospital from Jun.2020 to Jan.2023 were selected as the infection group,and 47 Hp-negative gastric cancer patients during the same period were selected as the control group.The levels of serum inflammatory factors IL-1β,IL-18,TNF-α,and Caspase-1 were measured by enzyme-linked im-munosorbent assays.And levels of mRNA and protein expression of NF-κB p56,NLRP3,and Caspase-1 were de-tected by fluorescent quantitative polymerase chain reaction and Western blot methods,respectively.RESULTS The levels of peripheral blood inflammatory factors IL-1β,IL-18,TNF-α,and Caspase-1 in the infection group were higher than those in the control group,and the mRNA expression and relative protein expression of NF-κB p56,NLRP3,and Caspase-1 in the peripheral blood mononuclear cells were higher than those in the control group(P＜0.05);the expression of NF-κB p56,NLRP3,and Caspase-1 protein in the infection group and the control group of stage Ⅲ was higher than those in the control group of stage Ⅰ-Ⅱ,and the protein expression elevated with the increase of pathological stage(P＜0.05).CONCLUSION Helicobacter pylori infection promoted the acti-vation of the NF-κB/NLRP3 signaling pathway and the release of inflammatory factors such as IL-1β and IL-18,which were closely related to the occurrence of gastric cancer.
Helicobacter pyloriGastric cancerNuclear factor κBNod-like receptor family protein 3Cysteine aspartic acid protein-1Interleukin-1β
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