Effect of salvia miltiorrhiza extracts on level of inflammation in rats with infected bone defects
OBJECTIVE To investigate the effect of salvia miltiorrhiza extracts on the level of inflammation in rats with infected bone defects.METHODS Fifty SPF-grade SD rats were selected,10 rats were used as the control group,and another 40 rats were modeled as infected bone defects.Thirty rats with successful modeling were divid-ed into the model group,the drug control group,and the radix salviae miltiorrhizae extract group,with 10 rats in each group.The pathological histology,bone mineral density,bone ash content,levels of vascular endothelial growth factor(VEGF),bone growth factor,inflammatory factors,expression of calcitonin gene-related peptide(CGRP)/phosphatidylinositol 3-kinase(PI3K)/serine threonine protein kinase(Akt)pathway-related mRNA ex-pression and CGRP)/PI3K/Akt pathway in the rats of all the groups were observed.RESULTS Compared with the control group,bone mineral density,bone ash content,vascular endothelial growth factor(VEGF),insulin-like growth factor-1(IGF-1),basic fibroblast growth factor(FGF-2),CGRP,PI3K,Akt mRNA expression,CGRP,PI3K,Akt expression were decreased in the model group,the drug control group,and the Radix Salviae Miltior-rhizae extract group,while the interleukin-10(IL-10),tumor necrosis factor-a(TNF-a),and nuclear factor κB(NF-κB)were increased(P＜0.05).Compared with the model group,the bone mineral density,bone ash con-tent,VEGF,IGF-1,FGF-2,CGRP,PI3K,Akt mRNA expression,CGRP,PI3K,Akt expression levels in the medication control group and the Radix Salviae Miltiorrhizae extract group were increased,while IL-10,TNF-αand NF-κB were decreased(P＜0.05).Compared with the drug control group,the bone mineral density,bone ash content,VEGF,IGF-1,FGF-2,CGRP,PI3K,Akt mRNA expression,CGRP,PI3K,Akt expression were in-creased in the Radix Salviae Miltiorrhizae extract group,while IL-10,TNF-α and NF-κB were decreased(P＜0.05).CONCLUSION The intervention of salvia miltiorrhiza extract on rats with infected bone defects elevated the expression of vascular endothelial growth factor and bone growth factor in rats,attenuated the level of inflamma-tory factors,and promoted the recovery of rats,and its mechanism might be related to the regulation of CGRP/PI3K/Akt pathway.
Infected bone defectsSalvia miltiorrhiza extractRatInflammatory factorsVascular endothelial growth factor
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