Intervention effect and mechanisms of forsythia extracts on rats with Streptococcus pneumoniae infection-induced acute exacerbation of chronic obstructive pulmonary disease
OBJECTIVE To explore the intervention mechanisms of forsythia extracts on rats with Streptococcus pneumoniae infection-induced acute exacerbation of chronic obstructive pulmonary disease(AECOPD).METHODS Totally 40 SD rats of SPF grade were randomly divided into the normal group,the model group,the low-dose forsythia extract group and the high-dose forsythia extract group,with 10 rats in each group.S.pneu-moniae was chosen to establish the S.pneumoniae infection-induced AECOPD rat models,the control group was not given any treatment.The intervention was carried out after the modeling was done,the ratio of tube wall area to total vessel area(WA％)and the ratio of muscular artery wall thickness to outer diameter of vessel(WT％)were measured.The levels of pulmonary function indexes and inflammatory factors as well as the relative expres-sion levels of mucoprotein 5AC(MUC5AC),epidermal growth factor receptor(EGFR)and phosphorylation-mito-gen-activated protein kinase p38 antibody(p38MAPK)in lung tissues were observed.RESULTS The WT％,WA％,tumor necrosis factor-α(TNF-α)level,interleukin(IL)-6 level,IL-8 level,and the expression levels of p38MAPK,MUC5AC and EGFR were higher in the model group,the low-dose forsythia extract group and the high-dose forsythia extract group than in the control group;the peak expiratory flow(PEF),forced vital capacity(FVC)and forced expiratory volume in one second(FEV1)were lower in the model group,the low-dose forsythia extract group and the high-dose forsythia extract group than in the control group(P＜0.05).The expression levels of PEF,FEV1 and FVC of the low-dose forsythia extract group and the high-dose forsythia extract group were higher than those of the model group;while the WA％,WT％,IL-6 level,IL-8 level,TNF-α level,MUC5AC level,p38MAPK level and EGFR level of the low-dose forsythia extract group and the high-dose forsythia extract group were lower than those of the model group(P＜0.05);the expression levels of PEF,FVC and FEV1 of the high-dose forsythia extract group were higher than those of the low-dose forsythia extract group,while the WA％,WT％,TNF-α level,IL-8 level,IL-6 level,EGFR level,MUC5AC level and p38MAPK level of the high-dose forsythia extract group were lower than those of the low-dose forsythia extract group(P＜0.05).CONCLUSION The forsythia extracts may effectively improve the pulmonary function of the rats with S.pneumo-iae infection-induced AECOPD,inhibit the expression of inflammatory factors,regulate the vascular remodeling of the lungs and improve the prognosis.
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