Association between postoperative infection in colon cancer patients and cellular immunity and intestinal flora
OBJECTIVE To explore the relationship between change of cellular immunity indexes and composition and diversity of intestinal flora in the colon cancer(CC)chemotherapy patients with postoperative infection.METHODS Totally 107 colon cancer patients who underwent radical surgery and received chemotherapy in the 903th Hospital of the PLA Joint Logistic Support Force from Jan 2020 to Jan 2022 were enrolled in the study and were divided into the infection group with 23 cases and the non-infection group with 84 cases according to the sta-tus of infection during the chemotherapy.The clinical data of the enrolled patients were retrospectively analyzed.The sputum,midstream urine,stool and skin wound secretion specimens were collected by aseptic approach be-fore the chemotherapy and after the chemotherapy for 3 months,the pathogens were isolated and identified.The fresh stool specimens were collected for the first time after the chemotherapy for 3 months,the intestinal flora were detected.The expression levels of CD3+,CD4+and CD8+were detected by flow cytometry,the ratio of CD4+to CD8+was calculated.The clinical data,cellular immunity indexes,composition and diversity of intestinal flora were observed and compared between the infection group and the non-infection group before and after the chemotherapy.RESULTS The CD3+and CD4+levels and the ratio of CD4+to CD8+were lower in the infection group than in the non-infection group after the chemotherapy,while the CD8+level of the infection group was higher than that of the non-infection group(P＜0.05).The abundance and diversity indexe of Escherichia coli,Enterococcus and intestinal flora of the two groups were higher after the chemotherapy than before the chemother-apy,the abundance and diversity indexe of Lactobacillus and Bifidobacterium of the two groups were lower after the chemotherapy than before the chemotherapy.The abundance and diversity index of E.coli,Enterococcus and intestinal flora of the infection group were higher than those of the non-infection group,while the abundance and diversity index of Lactobacillus and Bifidobacterium of the infection group were lower than those of the non-infec-tion group(P＜0.05).CONCLUSION The CC chemotherapy patients with postoperative infection show the decline of cellular immunity indexes,reduction of abundance of beneficial intestinal flora,rise of abundance of pathogenic flora and the decline of diversity of intestinal flora.
Colon cancerPostoperative chemotherapyInfectionCellular immune functionComposition and di-versity of intestinal flora
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