摘要
目的 分析高危型人乳头瘤病毒(HPV)感染与孕妇血清微小RNA(miR)-222、miR-21、miR-18a表达的关联.方法 选取2019年9月-2022年6月济南市第二妇幼保健院收治的高危型HPV感染孕妇102例为高危型HPV感染组,同期HPV感染阴性孕妇124名为对照组.分析两组一般资料,高危型HPV类型(HPV16、HPV18、HPV31、HPV33、HPV51、HPV52、HPV53 及 HPV58),两组血清 miR-222、miRNA-21、miR-18a 表达水平、宫颈阴道微生态菌群、母婴结局.结果 高危型HPV感染组、对照组年龄、孕前体质量指数(BMI)、产妇类型、孕次、检测时孕周、合并妊娠期疾病等一般资料比较,无统计学差异.102例高危型HPV类型主要为HPV16,占比54.90％,其次为HPV18,占比20.59％.与对照组比较,高危型HPV感染组血清miR-222、miR-NA-21、miR-18a表达水平更高(P＜0.05).与对照组比较,高危型HPV感染组乳酸杆菌阳性比例更低,假丝酵母、加德纳细菌阳性比例更高(P＜0.05).高危型HPV感染组自然流产、早产、总不良母婴结局发生率高于对照组(P＜0.05).结论 高危型HPV感染主要类型为HPV16、HPV18,能增加孕妇血清miR-222、miRNA-21、miR-18a表达水平,影响宫颈阴道微生态菌群,威胁母婴结局.
Abstract
OBJECTIVE To analyze the association between the high-risk human papillomavirus(HPV)infection and the expressions of serum micro ribonucleic acid-222(miR-222),micro ribonucleic acid-21(miR-21)and micro ribonucleic acid-18a(miR-18a)in pregnant women.METHODS A total of 102 pregnant women with high-risk HPV infection who were treated in Jinan Second Maternal and Child Health Hospital from Sep 2019 to Jun 2022 were assigned as the high-risk HPV infection group,meanwhile,124 pregnant women who did not have HPV in-fection were chosen as the control group.The baseline data of the two groups were analyzed,the subtypes of high-risk HPV(HPV16,HPV18,HPV31,HPV33,HPV51,HPV52,HPV53 and HPV58)were observed,the ex-pression levels of serum miR-222,miRNA-21 and miR-18a,cervical and vaginal microflora,and maternal and in-fant outcomes were observed and compared between the two groups.RESULTS There were no significant differ-ences in the baseline data such as age,progestational body mass index(BMI),types of puerpera,gravidity,ges-tational weeks at detection and complication with gestational diseases between the high-risk HPV infection group and the control group.Among the 102 pregnant women with high-risk HPV infection,the pregnant women with HPV16 infection accounted for 54.90％,followed by the pregnant women with HPV18 infection(20.59％).The expression levels of serum miR-222,miRNA-21 and miR-18a of the high-risk HPV infection group were higher than those of the control group(P＜0.05).The proportion of pregnant women who were positive for Lactobacillus was lower in the high-risk HPV infection group than in the control group,while the proportion of pregnant women who were positive for Candida and Gardnerella were higher in the high-risk HPV infection group than in the con-trol group(P＜0.05).The incidence rates of spontaneous abortion,premature delivery,adverse maternal and in-fant outcomes in total were higher in the high-risk HPV infection group than in the control group(P＜0.05).CONCLUSION HPV16 and HPV18 are the major high-risk subtypes of HPV causing the infection,which increase the expression levels of serum miR-222,miRNA-21 and miR-18a,affect the cervical and vaginal microflora,and pose threats to the maternal and infant outcomes.
基金项目
吉林省财政厅省级部门预算科学研究基金资助项目(2019-2021)()
NETL
NSTL
万方数据