Pathogens isolated from bronchoalveolar lavage fluid of children with lobar pneumonia,drug resistance and risk factors
OBJECTIVE To analyze the distribution and drug resistance of pathogens isolated from bronchoalveolar lavage fluid(BALF)of the children with lobar pneumonia and observe the risk factors.METHODS A total of 200 children with lobar pneumonia who were treated in Jingzhou Hospital Affiliated to Yangtze University from Jul 2018 to Aug 2021 were assigned as the study group,the distribution and drug resistance of pathogenic microor-ganisms isolated from BALF were observed,the patients of the study group were divided into the mild group,the moderate group and the severe group according to the severity of pneumonia.Meanwhile,110 children who had other types of respiratory tract infections were chosen as the control group.The expression levels of serine-threo-nine kinase(Akt)and nuclear factor-κB(NF-κB)in peripheral blood mononuclear cells(PBMCs)were detected.The changes of levels of serum white blood cells(WBC),C-reactive protein(CRP),interleukin(IL)-6,IL-1 β and tumor necrosis factor-α were monitored.RESULTS Totally 214 strains of pathogens were isolated from BALF of the patients in the study group,Mycoplasma pneumoniae was the predominant species of pathogen,followed by Streptococcus pneumoniae;the drug resistance rates of the two species of pathogens were respectively highest to levofloxacin and penicillin.The age and body mass index of the study group were significantly lower than those of the control group,the expression levels of Akt,NF-κB and inflammatory factors of the study group were signifi-cantly higher than those of the control group(P＜0.05).Akt and NF-κB were the risk factors for the lobar pneu-monia,while the age was the protective factor(P＜0.05).Among the children of the study group,the expression levels of Akt,NF-κB and inflammatory factors of the severe group were significantly higher than those of the moderate group and the mild group,and the expression levels of the above indexes of the moderate group were significantly higher than those of the mild group(P＜0.05).CONCLUSION M.pneumoniae is the predominant spe-cies of pathogen isolated from the children with lobar pneumonia,followed by S.pneumoniae and adenovirus,and the mixed infections of the pathogens also take a certain proportion.The onset of the lobar pneumonia shows a younger-age trend.The activation of Akt/NF-κB signaling pathways and high expressions of inflammatory factors are closely associated with the progression of illness condition.
Lobar pneumoniaSpectrum of pathogensSerine-threonine kinaseNuclear transcription factor-κBInflammatory factorDrug resistance
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