Effect of Polygonum capulosus extract on rats infected with Escherichia coli pyelonephritis by regulating PI3K/Akt signaling pathway
OBJECTIVE To analyze the intervention effect of Polygonum capulosus extract on Escherichia coli pye-lonephritis rats by regulating phosphatidylinositol 3 kinase/protein kinase B(PI3K/Akt)signaling pathway.METHODS Forty-five SPF Wistar rats were selected and 10 rats were in the control group and the remaining 35 rats were used to establish the model of pyelonephritis infected with E.coli.A total of 30 rats were successfully established as the model.They were divided into three groups including the model group,the drug control group,and the Persicaria capitata extract group,with 10 rats in each group.The control group was given levofloxacin in-tragastric administration.Polygonum capitatum extract group was given by intragastric administration.The control group and model group were given 0.5 ml normal saline intragastric administration without treatment,and the changes of the rats in each group were observed after intervention for 7 days.RESULTS Compared with the control group,Interleukin(IL)-6,IL-4,IL-10,secretory immunoglobulin A(SIgA),serum creatinine(Scr),Blood urea nitrogen(BUN),24-hour Proteinuria,PI3K,AKT expression in the model group,drug control group,and Per-sicaria capitata extract group increased(P＜0.05).Compared with model group,SIg A,IL-4,IL-6,IL-10,BUN,24h proteinuria,Scr,P13K and AKT expression levels in the drug control group and Polygonum capuifoli-um extract group were decreased(P＜0.05).Compared with the drug control group,the expression levels of IL-6,IL-10,IL-4,SIg A,BUN,Scr,24h proteinuria,AKT and PI3K in the Polygonum capitatum extract group were decreased(P＜0.05).CONCLUSION The intervention of Polygonum capuiflorum extract in E.coli infected pyelonephritis rats can effectively reduce the inflammatory response,promote the secretion of SIg A by the im-mune system,protect the kidney tissue of rats,and slow down the kidney injury.
Escherichia coliInfectionPyelonephritisPolygonum capuiflorum extractPhosphatidylinositol 3 kinase/protein kinase B
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