首页|慢性牙周炎患者牙龈卟啉单胞菌感染与NLRP-3炎性小体通路及HMGB1和MMP-3的关联

慢性牙周炎患者牙龈卟啉单胞菌感染与NLRP-3炎性小体通路及HMGB1和MMP-3的关联

Correlation of Porphyromonas gingivalis infection with NLRP-3 inflammatory body pathway,HMGB1 and MMP-3 in patients with chronic periodontal disease

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目的 探讨慢性牙周炎患者牙龈卟啉单胞菌感染与Nod样受体蛋白-3(NLRP-3)炎性小体通路、高迁移率族蛋白1(HMGB1)、基质金属蛋白酶-3(MMP-3)的关联.方法 回顾性收集2021年1月-2023年3月衡水市人民医院收治的103例慢性牙周炎患者的临床资料为病例组,根据所选患者是否感染牙龈卟啉单胞菌将其分为感染组(25例)和未感染组(78例),同时根据所选患者的病情严重程度将其分为轻度组(43例)、中度组(32例)和重度组(28例),另选115名同期收治的牙周健康者的临床资料为对照组.比较病例组和对照组牙龈卟啉单胞菌感染阳性率,病例组和对照组、不同病情严重程度慢性牙周炎患者、感染组和未感染组牙周膜组织NLRP-3炎性小体通路相关指标及血清HMGB1、MMP-3水平,用Pearson相关性分析慢性牙周炎患者牙龈卟啉单胞菌感染与牙周膜组织NLRP-3炎性小体通路相关指标及血清HMGB1、MMP-3水平的关系.结果 病例组牙龈卟啉单胞菌感染阳性率为24.27％,高于对照组的11.30％(P＜0.05);病例组牙周膜组织NLRP-3信使核糖核酸(mR-NA)、半胱氨酸天冬氨酸蛋白酶-1(Caspase-1)mRNA、接头蛋白凋亡相关斑点样蛋白(ASC)mRNA及血清HMGB1、MMP-3 水平高于对照组(P＜0.05);重度组牙周膜组织 NLRP-3 mRNA、Caspase-1 mRNA、ASC mR-NA及血清HMGB1、MMP-3水平高于中度组和轻度组(P＜0.05),中度组上述指标高于轻度组(P＜0.05);感染组牙周膜组织NLRP-3 mRNA、Caspase-1 mRNA、ASC mRNA及血清HMGB1、MMP-3水平高于未感染组(P＜0.05);Pearson相关性分析结果显示,慢性牙周炎患者牙龈卟啉单胞菌感染与牙周膜组织NLRP-3 mR-NA、Caspase-1 mRNA、ASC mRNA及血清HMGB1、MMP-3水平呈正相关(P＜0.05).结论 慢性牙周炎患者牙周膜组织NLRP-3 mRNA、Caspase-1 mRNA、ASC mRNA及血清HMGB1、MMP-3水平呈高表达,且其参与患者病情进展过程,并与牙龈卟啉单胞菌感染密切相关.
OBJECTIVE To investigate the correlation of porphyromonas gingivalis infection with nod like receptor protein-3(NLRP-3)inflammatory body pathway,high-mobility group protein 1(HMGB1)and matrix metallo-proteinase-3(MMP-3)in patients with chronic periodontal disease.METHODS The clinical data of 103 patients with chronic periodontitis admitted to Hengshui city people's hospital from Jan 2021 to Mar 2023 were retrospec-tively collected as the case group.The patients were divided into the infection group(25 cases)and the non-infec-tion group(78 cases)according to whether they were infected with Porphyromonas gingivalis,and they were al-so divided into the mild group(43 cases),the moderate group(32 cases)and the severe group(28 cases)accord-ing to the severity of the disease.The clinical data of 115 periodontally healthy people were collected as the control group.The positive rate of P.gingivalis infection between the case group and the control group was com-pared.The levels of periodontal ligament tissue NLRP-3 inflammatory pathway related indicators and serum HMGB1 and MMP-3 between the case group and the control group,among the chronic periodontitis patients with different severity,and between the infected group and the non-infected group were all compared.The correlation of P.gingivalis infection with periodontal ligament tissue NLRP-3 inflammatory pathway related indicators and ser-um HMGB1 and MMP-3 levels were analyzed by Pearson correlation.RESULTS The positive rate of P.gingival-is infection was 24.27％in the case group,which was higher than that in the control group(11.30％)(P＜0.05).The messenger ribonucleic acid(mRNA)levels of periodontal ligament tissue NLRP-3,Caspase-1 and ASC and serum HMGB1 and MMP-3 in the cases group were higher than those in the control group(P＜0.05).The levels of periodontal ligament tissue NLRP-3 mRNA,Caspase-1 mRNA,ASC mRNA and serum HMGB1 and MMP-3 in the severe group were higher than those in the moderate and the mild group(P＜0.05);the above in-dexes in the moderate group were higher than those in the mild group(P＜0.05).The levels of periodontal liga-ment tissue NLRP-3 mRNA,Caspase-1 mRNA,ASC mRNA and serum HMGB1 and MMP-3 in the infected group were significantly higher than those in the non-infected group(P＜0.05).Pearson correlation analysis showed that P.gingivalis infection was positively correlated with levels of periodontal ligament tissue NLRP-3 mRNA,Caspase-1 mRNA,ASC mRNA and serum HMGB1 and MMP-3(P＜0.05).CONCLUSION The ex-pression levels of periodontal ligament tissue NLRP-3 mRNA,Caspase-1 mRNA,ASC mRNA and serum HMGB1 and MMP-3 were higher in patients with chronic periodontitis,and they were involved in the process of disease progression and closely related to P.gingivalis infection.

Chronic periodontal diseasePorphyromonas gingivalisInfectionNod like receptor protein-3High-mobility group protein 1Matrix metalloproteinase-3

蔡晨雨、王林、李静、夏英杰、李媛媛

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衡水市人民医院口腔正畸修复科,河北衡水 053000

慢性牙周炎 牙龈卟啉单胞菌 感染 Nod样受体蛋白-3 高迁移率族蛋白1 基质金属蛋白酶-3

河北省卫健委医学科学研究项目衡水市2021年度科技计划自筹经费项目

202204612021014093Z

2024

10.11816/cn.ni.2024-231069

中华医院感染学杂志
中华预防医学会 中国人民解放军总医院

中华医院感染学杂志

CSTPCD北大核心
影响因子：1.885
ISSN：1005-4529
年,卷(期)：2024.34(7)
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