Expressions of cGAS/STING/TBK1 signaling pathway related genes and risk factors for HPV infection in patients with cervical lesions
OBJECTIVE To investigate the expression of cyclic guanine nucleotide adenine nucleotide synthetase/in-terferon activated protein/TANK binding kinase 1(cGAS/STING/TBK1)signaling pathway related genes in HPV infection in patients with cervical lesions and its susceptibility factors.METHODS The clinical data of 151 cases of Human papillomavirus(HPV)infected cervical lesions treated in Ji'an Central People's Hospital from Mar 2019 to Mar 2023 were retrospectively analyzed as the study group,and the clinical data of 151 cases of HPV-negative cervical lesions during the period were included in the control group.The clinical data between the two groups were compared,and the predisposing factors of HPV infection in patients with cervical lesions were ana-lyzed by univariate and multi-variate analysis.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the mRNA expression levels of cGAS,STING and TBK1,which were compared be-tween the study group and the control group.Receiver operating characteristic curve(ROC)was used to analyze the diagnostic value of individual and combined detection of cGAS,STING and TBK1 for HPV infection in pa-tients with cervical lesions.RESULTS Multivariate logistic regression analysis showed that older age,history of cervicitis,high frequency of sexual life,no use of condoms,no vulvar cleaning before and after sexual life,age 20 years of first sexual life,family history of cervical cancer,junior high school or below,and number of sexual partners ≥2 were all predisposing factors for HPV infection in patients with cervical lesions(P＜0.05).The mR-NA expression levels of cGAS,STING and TBK1 in the study group were higher than those in the control group(P＜0.05).The area under curve(AUC)of combined detection of cGAS,STING and TBK1 levels in the diagno-sis of HPV infection in patients with cervical lesions was 0.902,which was higher than that of individual detection(P＜0.05).The sensitivity and specificity of combined detection were 82.80％and 82.10％,respectively,with high diagnostic value.CONCLUSION The cGAS/STING/TBK1 signaling pathway was activated after HPV infec-tion in patients with cervical lesions.Combined detection of cGAS,STING and TBK1 is helpful for the diagnosis of HPV infection in patients with cervical lesions.HPV infection in patients with cervical lesions is related to many factors such as age,history of cervicitis,and frequency of sexual life.
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