Effect of the expression of TLRs related inflammatory factors on cervical cancer induced by human papillomavirus infection
OBJECTIVE To investigate the role of the expression of Toll-like receptors(TLRs)mediating related in-flammatory factors involved in cervical cancer induced by human papillomavirus(hr-HPV)infection.METHODS A total of 170 patients with hr-HPV infection in Xingtai People's Hospital from Jan 2023 to Dec 2023 were en-rolled in the study.According to the varying degrees of cervical lesions,they were divided into the cervicitis group(50 cases),low-grade squamous intraepithelial lesions(LSIL)group(50 cases),and high-grade squamous intra-epithelial lesions(HSIL)group(50 cases).The expression of TLRs signaling pathway related genes,including in-terleukin(IL)-6,IL-10,tumor necrosis factor(TNF-α)and high mobility group protein 1(HMGB1)were detec-ted in cervical tissues of 20 patients with cervical cancer.RESULTS Compared with the cervicitis group,the ex-pression levels of TLR4,TLR7,TLR9,nuclear transcription factor(NF-κB),IL-6,IL-10,TNF-α and HMGB1 in the LSIL group,HSIL group and cervical cancer group increased to varying degrees,while the expression levels of TLR3 and IFN-β decreased(P＜0.05).Compared with the LSIL group,the expression levels of TLR4,TLR7,TLR9 and NF-κB genes in the HSIL group and cervical cancer group increased to different extent,while the ex-pression levels of TLR3 and IFN-β genes decreased(P＜0.05).Compared with the HSIL group,the gene expres-sion of TLR4,TLR7,TLR9,NF-κB,the levels of IL-6,IL-10,TNF-α,HMGB1 and CD8+in the cervical cancer group increased,while the gene expression of TLR3 and IFN-β,the levels of CD3+,CD4+and CD4+/CD8+ de-creased(P＜0.05).Compared with the LSIL group,the levels of IL-6,IL-10,TNF-α,HMGB1 and CD8+in the HSIL group and cervical cancer group were increased to varying degrees,while the levels of CD3+,CD4+and CD4+/CD8+were decreased(P＜0.05).Compared with the cervicitis group,CD3+,CD4+and CD4+/CD8+in the LSIL group,HSIL group and cervical cancer group decreased to varying degrees,while CD8+increased to varying degrees(P＜0.05).CONCLUSION Hr-HPV may participate in the activation of cervical TLRs signaling pathway,mediate the secretion of related inflammatory factors,inhibit CD3+and CD4+differentiation,and pro-mote the progression of cervical diseases through cell-mediated immune response inhibition.
Toll-like receptorInflammatory factorsHuman papillomavirusCervical cancer
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