系统性红斑狼疮合并细菌性感染患者STAT1、STAT3、IL-1β表达与Th1/Th2平衡的相关性
Expressions of STAT1,STAT3 and IL-1β in systemic lupus erythematosus patients complicated with bacterial infection and association with balance of Th1/Th2
杜坤 1李秀琴 1杨友琴1
作者信息
- 1. 北大医疗海洋石油医院检验科,天津 300000
- 折叠
摘要
目的 探讨系统性红斑狼疮合并细菌性感染患者信号转导激活因子(STAT)1、STAT3、白细胞介素(IL)-1βmRNA相对表达量及其与辅助性T细胞亚群1/2(Th1/Th2)平衡的关系.方法 选取2020年1月-2023年6月北大医疗海洋石油医院收治的30例系统性红斑狼疮合并细菌性感染患者和35例系统性红斑狼疮未合并细菌性感染患者,分别为感染组和非感染组,比较两组患者外周血Th1、Th2及STAT1、STAT3、IL-1β表达水平,分析STAT1、STAT3、IL-1β与Th1、Th2的相关性,受试者工作特征(ROC)曲线分析STAT1、STAT3、IL-1β对系统性红斑狼疮合并细菌性感染的诊断价值.结果 感染组Th1、Th1/Th2表达水平低于非感染组(P＜0.05),而Th2、STAT1、STAT3、IL-1β 表达水平高于非感染组(P＜0.05);STAT1、STAT3、IL-1β 与 Th1、Th1/Th2 呈负相关(P＜0.05),与Th2呈正相关(P＜0.05);STAT1、STAT3、IL-1β诊断系统性红斑狼疮合并细菌性感染的曲线下面积(AUC)为0.950,敏感度为86.67％,特异度为94.29％.结论 系统性红斑狼疮患者合并细菌性感染后,外周血 Th1、Th1/Th2 表达水平降低,Th2、STAT1、STAT3、IL-1β 表达水平升高;STAT1、STAT3、IL-1β 与Th1、Th1/Th2呈负相关,与Th2呈正相关;STAT1、STAT3、IL-1β联合对系统性红斑狼疮合并细菌性感染的诊断价值较高.
Abstract
OBJECTIVE To explore the relative expression levels of signal transduction activator(STAT)1,STAT3 and interleukin(IL)-1β messenger RNA(mRNA)in the systemic lupus erythematosus(SLE)patients complicated with bacterial infection and analyze the association with balance of helper T cell 1(Th1)/helper T cell 2(Th2).METHODS A total of 30 SLE patients with bacterial infection and 35 SLE patients without bacterial in-fection who were treated in Peking University Medical Offshore Oil Hospital from Jan 2020 to Jun 2023 were en-rolled in the study and were respectively assigned as the infection group and the non-infection group.The expres-sion levels of peripheral blood Th1,Th2,ST AT1,STAT3 and IL-1β were compared between the two groups of patients,the association between ST AT1,STAT3,IL-1β and Th1,Th2 was observed,and the values of STAT1,STAT3 and IL-1β in diagnosis of bacterial infection in the SLE patients were analyzed by means of receiver operat-ing characteristic(ROC)curves.RESULTS The expression levels of Th1 and Th1/Th2 of the infection group were significantly lower than those of the non-infection group(P＜0.05),while the expression levels of Th2,STAT1,STAT 3 and IL-1β of the infection group were significantly higher than those of the non-infection group(P＜0.05).The STAT1,STAT3 and IL-1β were negatively correlated with the Th1 and Th1/Th2(P＜0.05)but were positively correlated with Th2(P＜0.05).The area under curve(AUC)of STAT1,STAT3 and IL-1β was 0.950 in diagno-sis of bacterial infection in the SLE patients,with the sensitivity 86.67％,the specificity 94.29％.CONCLUSION The SLE patients complicated with bacterial infection show the decline of expression levels of peripheral blood Th1 and Th1/Th2 and the rise of expression levels of Th2,STAT1,STAT3 and IL-1β.STAT1,STAT3 and IL-1βare negatively correlated with Th1 and Th1/Th2 but were positively correlated with Th2.The joint detection of STAT1,STAT2 and IL-1β has high value in diagnosis of the bacterial infection in the SLE patients.
关键词
系统性红斑狼疮/细菌性感染/信号转导激活因子/白细胞介素-1β/受试者工作特征Key words
Systemic lupus erythematosus/Bacterial infection/Signal transduction activator/Interleukin-1β/Re-ceiver operating characteristic引用本文复制引用
基金项目
天津市教委自然科学科研计划(2023KJ013)
出版年
2024
NETL
NSTL
万方数据