摘要
目的 分析消化性溃疡患者幽门螺杆菌(Hp)不同菌型感染外周血T细胞亚群、胃肠激素及炎症因子水平.方法 选取安徽医科大学附属巢湖医院2020年1月-2022年12月收治的109例消化性溃疡患者为研究对象,采用免疫印迹法检测Hp菌株分型,分析Hp不同菌型感染患者外周血T细胞亚群CD3+、CD4+、CD8+和胃肠激素胃泌素(GAS)、胃动素(MTL)及炎症因子白细胞介素(IL)-17、IL-33、肿瘤坏死因子(TNF-α)水平.结果 109例消化性溃疡患者中Hp阳性患者为80例,阴性患者29例,Hp阳性率为73.39％;80例Hp阳性患者中HpⅠ型58例占73.50％,HpⅡ型22例占27.50％;与Hp阴性患者比较,感染Hp Ⅰ型、Hp Ⅱ型患者CD4+、CD4+/CD8+和 IL-17、IL-33、TNF-α 及 GAS、MTL 水平均升高(P＜0.05),且 HpⅠ 型感染患者高于 HpⅡ型感染患者(P＜0.05).结论 Hp不同菌型感染消化性溃疡患者外周血T细胞亚群、胃肠激素及炎症因子表达水平有统计学差异,对于区分消化性溃疡病情严重程度和及时治疗具有重要意义.
Abstract
OBJECTIVE To analyze the peripheral blood T cell subsets,gastrointestinal hormones and inflammatory factors of the peptic ulcer patients with different types of Helicobacter pylori(Hp)infections.METHODS Totally 109 peptic ulcer patients who were treated in Chaohu Hospital Affiliated to Anhui Medical University from Jan 2020 to Dec 2022 were recruited as the research subjects.The types of Hp strains were detected by Western Blot.The levels of peripheral blood T cell subsets CD3+,CD4+,CD8+,gastrointestinal hormones gastrin(GAS),motilin(MTL)and inflammatory factors interleukin(IL)-17,IL-33 and tumor necrosis factor(TNF-α)were observed and compared among the patients with different types of Hp infections.RESULTS Among the 109 peptic ulcer patients,80 were positive for Hp,29 were negative,with the positive rate of Hp 73.39％.Of the 80 Hp-positive patients,58 were type HpⅠ,accounting for 73.50％;22 were type HpⅡ,accounting for 27.50％.The levels of CD4+,CD4+/CD8+,IL-17,IL-33,TNF-α,GAS and MTL of the patients with type HpⅡ,HpⅡ infections were signifi-cantly higher than those of the Hp-negative patients(P＜0.05),and the levels of above indexes of the patients with type HpⅠ infection were significantly higher than those of the patients with type HpⅡ infection(P＜0.05).CONCLUSION There are significant differences in the expression levels of peripheral blood T cell subsets,gastrointestinal hor-mones and inflammatory factors among the patients with different types of Hp infections.The indexes have great significance in distinguishing the severity of peptic ulcer so as to carry out the treatment in a timely manner.
基金项目
安徽省高等学校自然科学研究项目(KJ2021A0337)
NETL
NSTL
万方数据