Action mechanisms and interventional effect of radices lithospermi extract on rats with bronchial asthma and pulmonary infection
OBJECTIVE To observe the action mechanisms and interventional effect of radices lithospermi extract on the rats with bronchial asthma and pulmonary infection.METHODS Totally 50 SPF grade SD rats were enrolled in the study,10 of which were assigned as the control group,the rest of 40 rats were established the bronchial asth-ma and pulmonary infection models,30 rats that were successfully established the models were divided into the model group,the drug control group and the radices lithospermi extract group,with 10 rats in each group.The pathological change of lung tissues of the rats were observed.The antioxidant status,inflammatory factors,lung function indexes and expression levels of extracellular signal regulated kinase/p38 Mitogen activated protein kinase(ERK/p38MAPK)pathway-related mRNA and proteins were detected and compared among the groups.RESULTS The levels of malondialdehyde(MDA),soluble triggering receptor expressed on myeloid cell-1(sTREM-1),procalcitonin(PCT)and interleukin-6(IL-6)and the expression levels of ERK/p38MAPK pathway-related mRNA and proteins were higher in the model group,the drug control group and the radices lithospermi extract group than in the control group(P＜0.05);the superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),forced expiratory volume in the first second(FEV1),forced vital capacity(FVC)and FEV1/FVC were lower in the model group,the drug control group and the radices lithospermi extract group than in the con-trol group(P＜0.05).The levels of MDA,sTREM-1,PCT and IL-6 and the expression levels of ERK/p38MAPK pathway-related mRNA and proteins were lower in the drug control group and the radices lithospermi extract group than in the model group(P＜0.05),while the SOD,GSH-Px,FEV1,FVC and FEV1/FVC of the drug control group and the radices lithospermi extract group were higher than those of the model group(P＜0.05).The levels of MDA,sTREM-1,PCT and IL-6 and the expression levels of ERK/p38MAPK pathway-relat-ed mRNA and proteins were lower in the radices lithospermi extract group than in the drug control group(P＜0.05),while the SOD,GSH-Px,FEV1,FVC and FEV1/FVC of the radices lithospermi extract group were high-er than those of the drug control group(P＜0.05).CONCLUSION Radices lithospermi extract can improve the antiox-idant status of the rats with bronchial asthma and pulmonary infection,reduce the levels of inflammatory factors and boost the lung function.The action mechanisms are associated with the ERK/p38 MAPK signaling pathways.
Bronchial asthma and pulmonary infectionRadices lithospermi extractLung functionInflammatory factor
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