Expression levels of miRNA-21/PTEN in cerebral infarction patients and risk factors
OBJECTIVE To explore the change of expression levels of micro RNA(miR)-21/phosphatase and ten-sin homologue protein(PTEN)in cerebral infarction patients complicated with pulmonary infection and analyze the risk factors.METHODS A total of 120 patients with cerebral infarction who were treated in Tianjin Fourth Central Hospital and Tianjin Hospital from Aug 2021 to Jan 2023 were recruited as the research subjects,33 of whom were complicated with pulmonary infection and were assigned as the infection group,and 87 were not com-plicated with pulmonary infection and were assigned as the non-infection group.The distribution of pathogens iso-lated from the cerebral infarction patients complicated with pulmonary infection and changes of expression levels of peripheral blood miR-21 and PTEN mRNA were observed,multivariate analysis was performed for the risk fac-tors for the pulmonary infection,and the risk prediction model was established.RESULTS Totally 34 strains of pathogens were isolated from the 33 patients complicated with pulmonary infection,26(76.47％)of which were gram-negative bacteria.The levels of peripheral blood miR-21 and PTEN mRNA of the infection group were sig-nificantly higher than those of the non-infection group(P＜0.05).Complication with diabetes mellitus,invasive treatment and bulbar paralysis were the independent risk factors for the pulmonary infection in the patients with cerebral infarction(OR=2.347;2.158;2.366,P＜0.05).The established regression model was as follows:Logit(P)=-8.647+diabetes mellitus×0.853+invasive treatment×0.769+bulbar paralysis×0.769.The receiver op-erating characteristic(ROC)curves for prediction of incidence of the pulmonary infection were drawn based on di-agnostic probability Logit(P),when Logit(P)was greater than 11,the area under curve(AUC)was 0.836,with the sensitivity 81.82％,the specificity 71.24％.CONCLUSION The incidence of pulmonary infection is high a-mong the patients with cerebral infarction,and the gram-negative bacteria are dominant.The cerebral infarction patients complicated with pulmonary infection show the rise of peripheral blood miR-21 and PTEN mRNA levels.The risk factors include the diabetes mellitus,invasive treatment and bulbar paralysis,and the prediction model established based on the risk factors has high predictive value.
Cerebral infarctionPulmonary infectionPathogenMicro RNA-21Phosphatase and tensin homo-logue proteinRisk factor
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