Mechanism of miR-203 targeting PI3K/Akt pathway to inhibit migration and invasion of hepatitis B virus-infected hepatocellular carcinoma cells
OBJECTIVE To investigate the mechanism of micrornA-203(miR-203)targeting phosphatidylinositol-3-kinase/protein kinase B(PI3K/Akt)pathway to inhibit the migration and invasion of hepatitis B virus-infected hepatocellular carcinoma cells.METHODS The expression of miR-203 in hepatocytes HL-7702 and hepatitis B vi-rus-infected hepatocellular carcinoma cells HepG2 was detected.HepG2 human hepatocellular carcinoma cells were divided into control group,silencing group and overexpression group after passage cultivation.The control group was human hepatitis B virus-infected hepatocellular carcinoma cells without any treatment,the silencing group was human hepatitis B virus-infected hepatocellular carcinoma cells transfected with miR-203 silencing plasmid,and the overexpression group was human hepatitis B virus-infected hepatocellular carcinoma cells transfected with miR-203 overexpression plasmid.The transfection efficiency of miR-203 was evaluated,and the effects of up-regulation of miR-203 on the migration,invasion,and MAPK pathway protein expression of human hepatitis B virus-infected hepatocellular carcinoma cells were analyzed.RESULTS Compared with normal hepatocytes HL-7702,the expres-sion of miR-203 in hepatitis B virus-infected hepatocellular carcinoma cell HepG2 was increased(P＜0.05).Com-pared with the control group,the expression levels of miR-203 and E-cadherin decreased,while the expression levels of matrix metalloproteinase-9(MMP-9),vascular endothelial growth factor(VEGF),matrix metalloprotei-nase-2(MMP-2),Slug,PI3K and Akt increased in the silencing group.The expression levels of miR-203 and E-cadherin in the overexpression group were increased,while the expression levels of MMP-9,VEGF,MMP-2,Slug,PI3K and Akt were decreased(P＜0.05).Compared with the silencing group,the expressions of miR-203 and E-cadherin in the overexpression group were increased,and the expressions of MMP-9,VEGF,MMP-2,Slug,PI3K and Akt were decreased(P＜0.05).CONCLUSION After the intervention of down-regulated miR-203,the number of migration and invasion of hepatitis B virus-infected hepatocellular carcinoma cells decreased,and the expression of migration-and invasion-related proteins was regulated,the mechanism of which might be related to the inhibition of PI3K/Akt pathway.
MicroRNA-203Targeting phosphatidylinositol-3-kinase/protein kinase BHepatitis B virus infec-tionMigrationInvasionLiver cancer
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