摘要
目的 分析宏基因组学下一代测序(mNGS)在湖南省流行病原体筛查检测中的临床应用价值,并分析不同流行病原体的流行趋势和临床特征.方法 收集整理华中区进行mNGS病原体检测的患者数据,统计高频检出病原体,并分析和追踪其流行趋势及易感特征.结果 共统计2019-2023年常见的流行病原体检测人群共16 627例,其中男性10 811例(65.02％),女性5 816例(34.98％);高频检出的病原体有新冠病毒、疱疹病毒(1、4、5型)、鲍氏不动杆菌、白假丝酵母、耶氏肺孢子菌、屎肠球菌、肺炎克雷伯菌、烟曲霉菌、铜绿假单胞菌、结核分枝杆复符合群、纹带棒状杆菌、肺炎链球菌、肺炎支原体、鹦鹉热衣原体;从2021年第四季度开始,对于病原体的检测样本逐年上升,呼吸科、重症科、感染科和儿科对于mNGS的接受度较高.结论 mNGS能缩短病原体检测时间,提高病原体识别率和准确率,可作为指导预防流行病学的应用依据.
Abstract
OBJECTIVE To analyze the clinical application value of metagenomics next generation sequencing(mNGS)in the screening and detection of epidemic pathogens in Hunan province,and to analyze the prevalence trends and clinical characteristics of different epidemic pathogens.METHODS The data of patients underwent mNGS pathogen detection in central China were collected and sorted out,the high-frequency detected pathogens were counted,and the prevalence trend and susceptibility characteristics were analyzed and tracked.RESULTS A total of 16 627 people were counted for common epidemic pathogens from 2019 to 2023,including 10 811 males(65.02％)and 5 816 females(34.98％).The pathogens detected at high frequency were SARS-COVID-19,her-pesvirus(type 1/4/5),Acinetobacter baumannii,Candida albicans,Pneumocystis yerinii,Enterococcus faeci-um,Klebsiella pneumoniae,Aspergillus fumigatus,Pseudomonas aeruginosa,Mycobacterium tuberculosis con-comitants,Corynebacterium striatum,Streptococcus pneumoniae,Mycoplasma pneumoniae,Chlamydia pecti and Bacillus coli.Starting from the fourth quarter of 2021,samples tested for pathogens were increased year by year,with the department of respiratory,intensive care,infectious disease,and pediatrics showing higher accept-ance of mNGS.CONCLUSION The mNGS could shorten the time of pathogen detection,improve the rate and ac-curacy of pathogen identification,and could be used as an application to guide preventive epidemiology.
基金项目
湖南省人民医院青年博士基金国自培育项目(2023)(BSJJ202218)
NETL
NSTL
万方数据