Influence of LncRNA PVT1 on apoptosis of human embryonic lung fibroblasts cells of patients with respiratory syncytial virus infection
OBJECTIVE To observe the influence of long-chain non-coding RNA(lncRNA)plasmacytoma variant translocation 1(PVT1)on damage of human embryonic lung fibroblasts(HELF)cells of the patients with respira-tory syncytial virus(RSV).METHODS A total of 38 asthma patients with RSV infection who were hospitalized in the First Peoples Hospital of Changzhou from Jan 2020 to May 2023 were enrolled in the study,and 38 healthy volunteers were also enrolled in the study.The expressions of lncRNA,PVT1 and miR-625-5p were detected by quantitative real-time polymerase chain reaction(qRT-PCR).The HELF cells were divided into the NC group(no infection),the RSV group(RSV infection),the si-NC+RSV group(transfected si-NC+RSV infection),the si-lncRNA PVT1+RSV group(transfection si-lncRNA PVT1+RSV infection),the miR-NC+RSV group(trans-fection miR-NC+RSV infection),the miR-625-5p+RSV group(transfection miR-625-5p mimic+RSV infec-tion),the anti-miR-NC+si-lncRNA PVT1+RSV group(cotransfection of anti-miR-NC and si-lncRNA PVT1+RSV infection)and the anti-miR-625-5p+si-lncRNA PVT1+RSV group(cotransfection of anti-miR-625-5p and si-lncRNA PVT1+RSV).Cell apoptosis was detected by using flow cytometry,expressions of proteins were de-tected by Western Blot,the levels of tumor necrosis factor-α(TNF-α)and interleukin(IL)-6 were detected by en-zyme-linked immunosorbent assay.The dual luciferase report experiment was carried out to analyze the targeting relationship between the lncRNA PVT1 and the miR-625-5p.RESULTS The expression level of lncRNA PVT1 was higher in serum and HELF cells of the patients with RSV infection than in serum or control NC of the healthy people(all P＜0.05).The expression levels of Bax,Cleaved-caspase-3 protein,levels of TNF-α and IL-6,and ap-optosis rate of HELF were increased significantly among the patients with RSV infection(all P＜0.05).All the effects were mitigated after the lncRNA PVT1 was silent or the miR-625-5p was overexpressed(all P＜0.05).LncRNA PVT1 targeted and regulated the expression of miR-625-5p.The expression levels of Bax,cleaved-caspase-3 proteins,levels of TNF-α and IL-6,and apoptosis rate of HELF cells were significantly higher in the an-ti-miR-625-5p+si-lncRNA PVT1+RSV group than in the anti-miR-NC+si-lncRNA PVT1+RSV group(all P＜0.05).CONCLUSION The low expression of lncRNA PVT1 inhibit the apoptosis of HELF cells and inflammatory response of the patients with RSV infection.
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