首页|NLRP3/ACS/Caspase-1信号通路在儿童腺病毒肺炎引发心肌应激损伤中的作用

NLRP3/ACS/Caspase-1信号通路在儿童腺病毒肺炎引发心肌应激损伤中的作用

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目的 探讨NOD样受体蛋白3(NLRP3)/凋亡相关斑点样蛋白(ASC)/半胱氨酸蛋白酶1(Caspase-1)信号通路在儿童腺病毒肺炎引发心肌应激损伤中的作用.方法 选取2021年1月-2023年1月于南阳医学高等专科学校第一附属医院就诊的腺病毒肺炎患儿112例为研究对象,根据有无心肌损伤分为无心肌损伤组76例、心肌损伤组36例,检测并比较两组病毒滴度、心肌酶谱水平、炎性因子、氧化应激因子、NLRP3通路相关mRNA、蛋白表达水平.结果 病毒载量为4×100～6×100 copy/μg的患儿占比最高,无心肌损伤组和心肌损伤组分别为33例(43.42％)、28例(45.16％);与无心肌损伤组比较,心肌损伤组肌钙蛋白Ⅰ(cTnI)、肌红蛋白(Mb)、肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、乳酸盐脱氢酶(LDH)、门冬氨酸氨基转移酶(AST)表达水平高,白细胞(WBC)计数、超敏C-反应蛋白(hs-CRP)、降钙素原(PCT)、白细胞介素(IL)-1β、IL-18、转化生长因子β(TGF-β)水平高,丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肤过氧化物酶(GSH-Px)水平高(P＜0.05);NLRP3、ACS、Caspase-1的mRNA转录水平及蛋白相对表达水平高(P＜0.05).结论 血清NLRP3、ACS、Caspase-1水平与腺病毒肺炎导致的心肌损伤相关指标相关,腺病毒可能是通过NLRP3/ACS/Caspase-1信号通路的激活介导氧化应激水平,参与病理损伤进程.
Role of NLRP3/ACS/Caspase-1 signaling pathways in myocardial stress injury induced by adenovirus pneumonia in children
OBJECTIVE To explore the role of NOD-like receptor protein 3(NLRP3)/apoptosis-associated speck-like protein containing a CARD(ASC)/cysteinyl aspartate specific proteinase 1(Caspase-1)in myocardial stress injury induced by adenovirus pneumonia in the children.METHODS A total of children with adenovirus pneumonia who were treated in the First Affiliated Hospital of Nanyang Medical College from Jan 2021 to Jan 2023 were re-cruited as the research subjects and were divided into the no myocardial injury group with 76 cases and the myocar-dial injury group with 36 cases according to the status of myocardial injury.The virus titer,myocardial enzyme level,and expression levels of inflammatory factors,oxidative stress factors,NLRP3 pathway-related mRNA and proteins were detected and compared between the two groups.RESULTS The proportion of the children with viral load ranging between 4×100 and 6×100 copy/µg was the highest,and there were 33(43.42％)cases in the no myocardial injury group and 28(45.16％)cases in the myocardial injury group.The expression levels of cardiac troponin Ⅰ(cTnⅠ),myohemoglobin(Mb),creatine kinase(CK),creatine kinase-isozyme(CK-MB),lactate de-hydrogenase(LDH)and aspartate aminotransferase(AST)of the myocardial injury group were significantly high-er than those of the no myocardial injury group,the levels of white blood cell(WBC),high-sensitivity C-reactive protein(hs-CRP),procalcitonin(PCT),interleukin(IL)-1β,IL-18 and transforming growth factor β(TGF-β)of the myocardial injury group were significantly higher than those of the no myocardial injury group;the levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)of the myocardial injury group were higher than those of the no myocardial injury group(P＜0.05).The mRNA transcription levels of NLRP3,ACS and Caspase-1 and the relative expression levels of proteins were significantly higher(P＜0.05).CONCLUSION The levels of serum NLRP3,ACS and Caspase-1 are associated with the related factors for the my-ocardial injury induced by adenovirus pneumonia,and the adenovirus may mediate the oxidative stress level by ac-tivating NLRP3/ACS/Caspase-1 signaling pathways and participate in the process of pathological injury.

Adenovirus pneumonia in childrenMyocardial injuryOxidative stressNOD-like receptor protein 3/apoptosis-associated speck-like protein containing a CARD/cysteinyl aspartate specific proteinase 1

王晓荣、陈中慧、聂建军、李静、邵雪飞

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南阳医学高等专科学校第一附属医院儿科,河南南阳 473000

南阳医学高等专科学校第一附属医院药学部,河南南阳 473000

南阳医学高等专科学校第一附属医院检验科,河南南阳 473000

南阳医学高等专科学校,河南南阳 473000

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儿童腺病毒肺炎 心肌损伤 氧化应激 NOD样受体蛋白3/凋亡相关斑点样蛋白/半胱氨酸蛋白酶1

河南省医学科技攻关计划基金资助项目南阳市市级科技计划基金资助项目

2020060729KJGG186

2024

10.11816/cn.ni.2024-231835

中华医院感染学杂志
中华预防医学会 中国人民解放军总医院

中华医院感染学杂志

CSTPCD北大核心
影响因子：1.885
ISSN：1005-4529
年,卷(期)：2024.34(14)
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