Pathogenic drug resistance and expression levels of serum miR-5787,miR-133,Bax,and Bcl-2 in patients with acute cerebral infarction complicated by pulmonary infection
OBJECTIVE To explore the drug resistance of pathogenic bacteria in patients with acute cerebral infarc-tion and pulmonary infection(PI),and to analyze the expression levels of serum microRNA-5787(miR-5787),microRNA-133(miR-133),B-lymphomatoma-2 related X protein(Bax),and B-lymphomatoma-2(Bcl-2).METHODS A total of 82 patients with acute cerebral infarction combined with pulmonary infection admitted to the People's Hospital of Emeishan from Jan 2021 to Aug 2023 were recruited as the group A,and 90 patients with a-cute cerebral infarction without pulmonary infection during the same period were enrolled in the group B.The dis-tribution and drug resistance of pathogenic bacteria in the group A were analyzed.The expression levels of serum miR-5787,miR-133,Bax,Bcl-2,red blood cell immune parameters and levels of inflammatory factor between the two groups were compared.The correlation of serum levels of miR-5787,miR-133,Bax and Bcl-2 with red blood cell immune parameters and inflammatory factors were analyzed by Pearson correlation analysis.RESULTS A total of 132 pathogenic strains were isolated from 82 patients with acute cerebral infarction combined with PI.Gram-positive bacteria accounted for 34.85％,mainly Staphylococcus aureus.Gram-negative bacteria accounted for 61.36％,mainly Klebsiella pneumoniae,and fungi accounted for 3.79％.Klebsiella pneumoniae had high drug resistance to clindamycin,ciprofloxacin,and sulfamethoxazole/trimethoprim,with the drug-resistant rates of 55.17％;S.aureus had high drug resistance to amoxicillin,ampicillin,penicillin,ceftazidime,and ciprofloxacin.Compared with the group B,the levels of serum miR-5787,miR-133,and Bax in the group A were higher while the levels of serum Bcl-2 were lower(P＜0.05).The levels of whole blood immune adhesion inhibitory factor(FEIR),serum interleukin-17(1L-17),and tumor necrosis factor a(TNF-a)in the group A were higher than those in the group B.The levels of whole blood synergistic tumor red blood cell rosette rate(ATER),natural tumor red blood cell rosette rate(NTER),and serum IL-10 were lower than those in the group B(P＜0.05).Se-rum miR-5787,miR-133 and Bax were positively correlated with levels of whole blood FEIR,serum IL-17 and TNF-a,but negatively correlated with levels of whole blood ATER,NTER and serum IL-10.Serum Bcl-2 was negatively correlated with levels of whole blood FEIR,serum IL-17,TNF-a,but positively correlated with levels of whole blood ATER,NTER and serum IL-10(P＜0.05).CONCLUSION The main pathogen in patients with a-cute cerebral infarction combined with PI is Klebsiella pneumoniae.PI can lead to the imbalance of the pro-inflam-matory/antis-inflammatory mechanisms in the patients,inhibit red blood cell immune function.The expression changes of serum miR-5787,miR-133,Bax,and Bcl-2 are closely related to the patients'red blood cell immune and inflammatory indicators.
Cerebral infarctionPulmonary infectionPathogenic bacteriaDrug resistanceMicro ribonucleic acid-5787Microribonucleic acid-133B-lymphoblastoma-2 related X proteinB-lymphoblastoma-2
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