Helicobacter pylori isolates from biopsy tissues of gastric mucosa of chronic gastritis patients,drug resistance,drug resistance genes and virulence genes
OBJECTIVE To analyze characteristics of Helicobacter pylori infection,drug resistance,related resist-ance genes and virulence genes in gastric mucosa.METHODS A total of 598 patients with chronic gastritis who re-ceived gastroscopy in Longyou County Traditional Chinese Medicine Hospital from Oct 2020 to Nov 2022 were in-cluded in the study.According to the results of gastric mucosa biopsy,they were divided into the infection group with132 cases and the uninfected group with 466 cases.General data of the two groups were analyzed,and drug re-sistance of H.pylori to commonly used antibiotics,drug-resistant genes and virulence genes of H.pylori were analyzed.RESULTS There were no significant differences in age,sex,body mass index(BMI),degree of chronic inflammation,intestinal metaplasia and proportion of intraepithelial neoplasia between the two groups.The drug resistance rate of H.pylori to metronidazole was higher than 80％,and the drug resistance rates to furazolidone,tetracycline and amoxicillin were lower than 20％.The rates of mutation at A2143G of 23S ribosomal ribonucleic acid(23S rRNA)gene and N87K of deoxyribonucleic acid gyrase subunit A(gyrA)gene were higher in drug-re-sistant H.pylori strains(P＜0.05).The detection rates of H.pylori virulence genes cytotoxin-associated gene A(CagA),vacuola-ting cytotoxin A sla(VacA s1a)+vacuola-ting cytotoxin A m1(VacAml)and VacA s1a in 132 strains were top 3,accounting for 100.00％,46.97％and 18.94％,respectively.CONCLUSION The drug re-sistance rate of H.pylori strains of gastric mucosa to metronidazole is higher than that to furazolidone,tetracy-cline and amoxicillin.The main cause of antibiotic resistance is related to the mutations of drug resistance genes,such as 23S rRNA and gyrA.The most common virulence gene is CagA gene.Screening of these mutations might help the clinic to prevent antibiotic resistance and select appropriate antibiotics to eradicate H.pylori.
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