Correlation between TAZ gene polymorphisms and Helicobacter pylori infection
OBJECTIVE To study the associations of the single nucleotide polymorphisms(SNPs)of rs16861985,rs1055153,rs6783790 and rs12490239 in TAZ(transcriptional coactivator containing PDZ binding motif)gene with Helicobacter pylori(Hp)infection.METHODS A total of 470 blood samples of volunteers under normal physical examination in the First Affiliated Hospital of Baotou Medical College and Inner Mongolia Baotou Steel Hospital from 2008 to 2020 were collected.The infection of Hp by enzyme linked immunosorbent assay(ELISA)was detected,including 248 positive cases of Hp and 222 cases of negative for Hp.TAZ rs16861985,rs1055153,rs6783790,rs12490239 were genotyped by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Unconditional logistic regression was used to evaluate the associations of SNPs with Hp infection under codominant,dominant,recessive and overdominant genetic models.And the akaike information criterion(AIC)and bayesian information criterion(BIC)were used to determine the best genetic model.SHEsis online software was used to construct the haplotypes of TAZ rs16861985,rs1055153,rs6783790 and rs12490239,and to analyze the associations of haplotypes with Hp infection.Generalized multifactor dimensionality reduction(GM-DR)was used to analyze the interactions of TAZ gene polymorphisms in Hp infection.RESULTS TAZ rs16861985,rs1055153,rs6783790 and rs12490239 genotypes were not associated with Hp infection.The fourth-order interaction of TAZ rs16861985,rs1055153,rs6783790 and rs12490239 was associated with Hp infection(P＜0.05).CONCLUSION Polymorphisms of TAZ gene rs16861985,rs1055153,rs6783790 and rs12490239 have no major effect on Hp infection in Baotou Han population.The haplotypes constructed by TAZ rs16861985,rs1055153,rs6783790 and rs12490239 are not associated with Hp infection.TAZ gene rs16861985,rs1055153,rs6783790 and rs12490239 show interactive effects in Hp infection in Baotou Han population.
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