2020-2022年某儿童专科医院铜绿假单胞菌分离株耐药性及Ⅰ类整合子变化趋势
Changing trends of drug resistance and class Ⅰ integron of Pseudomonas aeruginosa isolates in a children's hospital from 2020 to 2022
钱扬会 1齐永志 2李晓燕 1刘晓菁 1薛娟1
作者信息
- 1. 北京京都儿童医院检验科,北京 102208
- 2. 解放军总医院第六医学中心,北京 100048
- 折叠
摘要
目的 检测临床分离铜绿假单胞菌耐药性及Ⅰ类整合子变化.方法 北京京都儿童医院2020年1月—2022年12月临床分离的铜绿假单胞菌标本根据采集时间被分为三组:2020年、2021年和2022年.采用法国生物梅里埃公司的VITEK-2 Compact研究其对12种常用抗菌药物的耐药性,采用聚合酶链式反应(PCR)扩增检测Ⅰ类整合子.结果 2020组、2021组和2022组分别检出铜绿假单胞菌为46、57和72株;铜绿假单胞菌对12种常用抗菌药物耐药性增长不明显,Ⅰ类整合子阳性菌检出率逐年增加,且Ⅰ类整合子阳性铜绿假单胞菌耐药性高于阴性菌(P＜0.05).结论 儿童专科医院铜绿假单胞菌耐药性较高,Ⅰ类整合子阳性铜绿假单胞菌检测比例增加明显.需要加强医院感染的检测和耐药菌监控,进一步规范抗菌药物使用.
Abstract
OBJECTIVE To investigate the drug resistance and class Ⅰ integron in clinical isolated Pseudomonas aeruginosa(PA).METHODS Clinically isolated PA specimens from Jingdu Children's Hospital of Beijing from Jan 2020 to Dec 2022 were divided into three groups according to the collection time(2020,2021 and 2022,re-spectively).VITEK-2 Compact(BioMérieux France)was used to study the drug resistance of 12 common antimi-crobials.Class Ⅰ integrons were detected by polymerase chain reaction(PCR)assay.RESULTS A total of 46,57 and 72 strains of PA were detected in the 2020,2021 and 2022 groups,respectively.There was no significant in-crease in PA resistance to 12 common antibiotics.The detection rate of class Ⅰ integron positive bacteria increased year by year,and the drug resistance of class Ⅰ integron-positive PA was higher than that of negative bacteria(P＜0.05).CONCLUSION The drug resistance of PA in children's hospital is high,with a significant increase in the proportion of class Ⅰ integron-positive PA.It is necessary to strengthen the detection of nosocomial infections and the monitoring of drug-resistant bacteria,and to further standardize the use of antibiotics.
关键词
儿童/铜绿假单胞菌/Ⅰ类整合子/耐药性/抗菌药物/医院感染Key words
Children/Pseudomonas aeruginosa/Class Ⅰ integron/Drug resistance to antibiotics/Antimicrobial drug/Nosocomial infections引用本文复制引用
基金项目
解放军总医院第六医学中心创新培育基金(CXPY201902)
出版年
2024
NETL
NSTL
万方数据