Anti-inflammatory effect of R-(+)-pulegone on drug-resistant bacteria in mice with abdominal cavity infection
OBJECTIVE To determine the median lethal concentration(LC50)and absolute lethal concentration(LC100)of drug-resistant bacteria in mice infected intraperitoneally,and to investigate the anti-inflammatory effect of R-(+)-pulegone on mice infected with drug-resistant bacteria in the abdominal cavity.METHODS Mice were randomly divided into the normal control group(NC group),the drug-resistant strain infection model group(Model group),the R-(+)-pulegone group(PU group,20 mg/kg),and the cefixime group(CFM group,26 mg/kg),with 10 mice in each group.Except for the NC group,other test groups were intervened for three consecutive days,once a day.The Model group was given 20 ml/kg edible oil orally,and the PU group and CFM group were given an equal volume of R-(+)-pulegone and cefixime,respectively.On the third day,one hour after administration,drug-resistant Escherichia coli(10 ml/kg)was intraperitoneally injected.After observation for 24 and 48 hours,orbital blood,peritoneal lavage fluid,and peritoneal tissue of the mice were collected.A fully auto-mated cell analyzer was used to count white blood cells in blood and abdominal cavity rinse fluid.Morphological changes of blood cells was observed by using Wright-Giemsa staining assay,and pathological changes in the ab-dominal tissue was detected by hematoxylin-eosin(HE)staining.RESULTS LC50 and LC100 of drug-resistant bacteria intraperitoneally inoculated in the mice were 1.50 X 108 CFU/ml and 2.25 X 108 CFU/ml,respectively.Compared with the NC group,there were diffuse inflammation,hyperemia and edema in the Model group,while the pathological changes in the PU group and CFM Group were not obvious.The WBC count in the PU group was significantly lower than that in the NC group,but not significantly different from that in the CFM Group and Model group.The WBC count in peritoneal lavage fluid in the PU group was lower than that in the Model group,while it was higher than that in the NC group,but not significantly higher than the CFM Group,which indicated that R-(+)-pulegone had anti-inflammatory effects on drug-resistant bacteria in the abdominal cavity(P＜0.05).HE results showed that the structure of peritoneal tissue of the PU and CFM groups was intact,with orderly ar-rangement of mesothelial cells.Compared with the NC group,a small amount of lymphocyte infiltration was ob-served in the peritoneal stroma,and the peritoneal tissue structure was more complete and the lymphocyte infiltra-tion was less in the Model group.This indicated that the R-(+)-pulegone had significant anti-infective effect on the infection of intraperitoneal drug-resistant E.coli.CONCLUSION The R-(+)-pulegone has a certain inhibitory effect on inflammation of abdominal cavity in mice infected with drug-resistant bacteria,which can provide a cer-tain research basis for pharmacological mechanism of anti-infection effects of R-(+)-pulegone.
NETL
NSTL
万方数据
