OBJECTIVE To study the distribution of Acinetobacter baumannii in the environment of intensive care unit(ICU)of medical institutions,and to analyze the correlation between drug resistant phenotypes and drug re-sistant genes,so as to provide data support for formulating infection control strategies.METHODS ICU environ-mental samples from 7 medical institutions were collected by cotton swab smear method from Jan 2019 to Dec 2019.The strains were identified by matter-assisted laser desorption ionization time-of-flight mass spectrometer.The drug susceptibility was tested by microbroth dilution method.Drug-resistant genes were obtained by whole genome sequencing.RESULTS A.baumannii widely existed on the enviromental surfaces of medical institutions.The total detection rate was 6.79％(114/1 680),with the highest proportion of 12.28％(14/114)on surfaces of the mattress directly touched by patients.The drug resistance rates of ceftazidime and ciprofloxacin were the high-est,both of which were 84.21％(96/114).The multi-drug resistance rate of 114 strains was 85.96％(98/114).The drug resistance rates to tigecycline and polymyxin B were 1.75％(2/114)and 0.88％(1/114),respectively.Whole genome sequencing results showed that 114 strains of A.baumannii carried 6 classes of 26 kinds of drug-resistant genes,and the highest carrying rate of β-lactam resistance genes was 99.12％(113/114),no quinolone resistance gene was carried.Multivariate logistic regression analysis showed that the resistant genes of bla OXA.23,blaOXA-66,aph(3")-Ib,aph(6)-Id,arm A,sul1,sul2 and blaTEM-1D were all positively correlated with their re-spective antibiotic resistance(P＜0.05).Tet(B)resistance gene had no significant correlation with tigecycline.CONCLUSION A.baumannenii is common in ICU environment of medical institutions,and the drug resistance sit-uation is severe.blaOXA-23,blaOXA-66,aph(3")-Ib,aph(6)-Id,armA,sul1,sul2 and blaTEM-1D are the main drug re-sistance genes.Tetracycline and quinolone antibiotic resistance phenotypes and genotypes are inconsistent,and the resistance mechanism is complex.
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