OBJECTIVE To investigate the management status of final rinsing water in endoscopy centers of various levels of medical institutions in Shanghai.The findings will provide a basis for improving water quality,establis-hing quality control standards,and implementing regulatory measures.METHODS During the Shanghai hospital infection quality control inspection in 2023,on-site research was conducted on the management of final rinsing wa-ter in the endoscopy centers of the inspected medical institutions.The research included the management of final rinsing water,sampling and testing methods and waterway disinfection methods.RESULTS Among the 106 medi-cal institutions under inspection,94 hospitals were equipped with endoscopy centers.Less than 30%of the medical institutions independently cleaned and disinfected diagnostic and therapeutic endoscopes in separate areas,and ap-proximately 15%of the endoscopy centers lacked independent purified water treatment systems.The proportions of using purified water in final rinsing were 85.7%,87.8%,and 90.9%in tertiary hospitals,secondary hospitals,and privately-run hospitals,respectively.The investigation found that 78.6%of tertiary hospitals,51.2%of sec-ondary hospitals,and 81.8%of privately-run hospitals conducted monthly sampling and monitoring of final rinsing water(P<0.05).The proportions of disinfecting pipelines by chlorine-based disinfectants were 14.3%,22.0%,and 18.2%in each level,and the proportions of using R2A agar plate for culture detection of purified water were 35.7%,36.6%,and 27.3%,respectively,with no significant difference among different levels of hospitals.CONCLUSION The current status of endoscope water management in Shanghai is not optimistic,including insuffi-cient cognition of management personnel,inadequate hospital attention,inconsistent clinical implementation,and insufficient regulatory efforts.Targeted training should be carried out in the future to reduce the risk of infection.