首页|上尿路结石腔道手术后泌尿系统感染Toll样受体信号通路水平变化

上尿路结石腔道手术后泌尿系统感染Toll样受体信号通路水平变化

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目的 研究上尿路结石腔道手术后泌尿系统感染Toll样受体(TLRs)介导的炎症信号通路相关因子水平变化.方法 随机选择2021年8月-2023年8月在金华市中心医院就诊的上尿路结石并行腔道取石手术术后发生泌尿系统感染患者30例为感染组,30例未发生感染患者为对照组,收集患者临床资料,分析炎性因子白细胞计数(WBC)、C-反应蛋白(CRP)、降钙素原(PCT)、白细胞介素(IL)-17、IL-10、单核细胞趋化蛋白(MCP)-1和转化生长因子-β(TGF-β)水平变化及TLRs信号通路mRNA、蛋白相对表达水平.结果 与对照组比较,感染组患者 WBC、CRP、PCT、IL-17、MCP-1 水平升高(P＜0.05),IL-10、TGF-β 表达水平降低(P＜0.05);TLR4、MyD88、NF-KB mRNA转录水平及蛋白相对表达水平升高(P＜0.05);Pearson相关分析结果显示,TLRs介导的炎症信号通路相关因子表达与WBC、CRP、PCT均呈正相关(P＜0.05).结论 上尿路结石腔道手术后泌尿系统感染患者TLRs介导的炎症信号通路相关因子表达水平升高,可为术后感染临床预防提供支持.
Changes of Toll-like receptor signaling pathways in upper urinary calculi patients with urinary system infection after luminal lithotomy
OBJECTIVE To observe the changes of Toll-like receptors(TLRs)-mediated inflammatory signaling pathway-related factors in the upper urinary calculi(UUC)patients with urinary system infection after luminal li-thotomy.METHODS A total of 30 patients with UUC who underwent luminal lithotomy in Jinhua Central Hospi-tal from Aug 2021 to Aug 2023 were assigned as the infection group,and 30 patients who did not have infection were chosen as the control group.The clinical data were collected from the patients.The levels of inflammatory factors white blood cell(WBC),C-reactive protein(CRP),procalcitonin(PCT),interleukin(IL)-17,IL-10,monocyte chemoattractant protein(MCP)and transforming growth factor-β(TGF-β)as well as the relative expres-sion levels of TLRs signaling pathway mRNA and proteins were observed.RESULTS The levels of WBC,CRP,PCT,IL-17 and MCP-1 of the infection group were higher than those of the control group(P＜0.05),while the expression levels of IL-10 and TGF-β of the infection group were lower than those of the control group(P＜0.05).The transcription levels of TLR4,MyD88 and NF-κB mRNA as well as the relative expression levels of proteins of the infection group were higher than those of the control group(P＜0.05).Pearson correlation analysis showed that the expressions of the TLRs-mediated inflammatory signaling pathway-related factors were positively correla-ted with WBC,CRP and PCT(P＜0.05).CONCLUSION The UUC patients who have urinary system infection af-ter luminal lithotomy show the rise of expression levels of TLRs-medicated inflammatory signaling pathway-relat-ed factors,which may support the clinical prevention of postoperative infection.

Toll-like receptorUpper urinary calculiPostoperative infectionUrinary system infectionCorrela-tion analysisInflammatory factorC-reaction proteinProcalcitoninInterleukin-17

唐霞燕、陈英、陈智洋、朱德胜、刘磊

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浙江大学医学院附属金华医院碎石科,浙江金华 321000

浙江大学医学院附属金华医院设备科,浙江金华 321000

浙江大学医学院附属金华医院泌尿外科,浙江金华 321000

浙江达恩医疗检测实验室,浙江金华 321017

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Toll样受体 上尿路结石 术后感染 泌尿系统感染 相关性分析 炎症因子 C-反应蛋白 降钙素原 白细胞介素-17

浙江省卫生健康委基金资助项目金华市中心医院中青年科研启动基金资助项目

2022RC285JY2020-2-11

2024

10.11816/cn.ni.2024-231718

中华医院感染学杂志
中华预防医学会 中国人民解放军总医院

中华医院感染学杂志

CSTPCD北大核心
影响因子：1.885
ISSN：1005-4529
年,卷(期)：2024.34(17)
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