摘要
目的 探讨单一、多重高危型人乳头瘤病毒(HR-HPV)感染女性下生殖道菌群微生态的临床特征.方法 选取2022年1月-2023年12月于武汉市中西医结合医院行宫颈HR-HPV筛查阳性的患者93例,根据HR-HPV感染情况分为单一 HR-HPV感染组72例和多重HR-HPV感染组21例,另选取HR-HPV阴性的受检者452例作为对照组,所有受检者采集宫颈脱落细胞标本,采用聚合酶链式反应(PCR)荧光探针法进行宫颈HR-HPV分型检测,采集受检者的阴道分泌物标本检测细菌性阴道炎(BV)、滴虫性阴道炎(TV)、外阴阴道假丝酵母菌病(VVC)、解脲脲原体(UU)、沙眼衣原体(CT)的感染情况.结果 多重HR-HPV感染组的白细胞计数阳性率、pH值＞4.5阳性率高于单一 HR-HPV感染组(P＜0.05);单一 HR-HPV感染组与多重HR-HPV感染组的BV、TV、UU、CT阳性率比较差异无统计学意义;菌群多样性异常、白细胞计数阳性、pH值＞4.5、BV阳性、TV阳性、UU阳性、CT阳性与多重HR-HPV感染具有相关性(P＜0.05);菌群多样性异常、TV阳性、UU阳性、CT阳性与单一 HR-HPV感染具有相关性(P＜0.05).结论 HR-HPV感染与下生殖道菌群微生态失调密切相关,且与BV、TV、UU、CT感染有关.多重HR-HPV感染较单一 HR-HPV感染更易增加白细胞计数以及阴道弱酸性改变.
Abstract
OBJECTIVE To explore the clinical characteristics of lower genital tract microbiota in female patients with single,multiple high-risk human papillomavirus(HR-HPV)infections.METHODS Totally 93 patients who were positive for screening of cervical HR-HPV in Wuhan Integrated Traditional Chinese and Western Medicine Hospital from Jan 2022 to Dec 2023 were enrolled in the study and were divided into the single HR-HPV infection group with 72 cases and the multiple HR-HPV infection group with 21 cases according to the status of HR-HPV infection,meanwhile,452 participants who were tested negative for HR-HPV were chosen as the control group.The cervical exfoliated cell samples were collected from all of the subjects.The subtypes of cervical HR-HPV were detected by polymerase chain reaction(PCR)fluorescence probe,the vaginal secretion specimens were collected to detect Bacterial vaginitis(BV),Trichomonas vaginitis(TV),Vulvovaginal candidiasis(VVC),Ureaplasma ure-alyticum(UU)infection and Chlamydia trachomatis(CT)infection.RESULTS The positive rate of white blood cell counts and positive rate of pH value greater than 4.5 were higher in the multiple HR-HPV infection group than in the single HR-HPV infection group(P＜0.05).There were no significant differences in the positive rates of BV,TV,UU and CT between the single HR-HPV infection group and the multiple HR-HPV infection group.There was association between the abnormal diversity of flora,positive white blood cell count,pH value greater than 4.5,positive BV,positive TV,positive UU and positive CT and the multiple HR-HPV infection(P＜0.05).There was association between the abnormal diversity of flora,positive TV,positive UU,positive CT and the sin-gle HR-HPV infection(P＜0.05).CONCLUSION The HR-HPV infection is closely associated with lower genital tract dysbacteriosis and is associated with BV,TV,UU and CT infections.The multiple HR-HPV infection is more likely to lead to the increase of white blood cell counts and change of vaginal faintly acid.
基金项目
武汉市卫生健康委科研基金资助项目(WX18D21)
维普
万方数据