Intestinal flora of bronchial asthma children with secondary pulmonary infection
OBJECTIVE To explore the structure and functional characteristics of intestinal flora of the bronchial asthma children with secondary pulmonary infection.METHODS A total of 29 bronchial asthma children with sec-ondary pulmonary infection who were treated in respiratory medicine department of Anhui Province Children's Hospital from Feb 2022 to Feb 2024 were assigned as the infection group,meanwhile,31 children with simple asthma who were hospitalized for treatment were chosen as the non-infection group.The levels of inflammatory cytokines including interleukin(IL)-1β,IL-2R,IL-6,IL-8,IL-10 and tumor necrosis factor-α(TNF-α)were de-tected for the two groups of children.Metagenomic high-throughput technique was employed to carry out the se-quencing for the intestinal flora from fresh stool specimens that were collected from the two groups of children.RESULTS The levels of inflammatory cytokines IL-1β,IL-2R,IL-6,IL-8 and TNF of the infection group were higher than those of the non-infection group(P＜0.05).The α diversity and β diversity of intestinal flora were remarkably lower in the infection group than in the non-infection group(P＜0.05).Bacteroides,Prevotella,Alis-tipes and Eubacterium were dominant among the bacteria on genus level;the relative abundance of flora Prevotel-la,Megamonas,Akkermansia and Tannerella was higher in the infection group than in the non-infection group;while the relative abundance of Parabacteroides,Dialister,Faecalibacterium,Ruminococcus and Alistipes was higher in the non-infection group than in the infection group.The major difference in the dominant functional genes between the two groups was the metabolism,including biodegradation and metabolism of allogenic material,ener-gy metabolism and lipid metabolism.CONCLUSION The bronchial asthma children with secondary pulmonary in-fection show the rise of serum inflammatory cytokines and change of diversity of intestinal microorganisms and species composition.
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