Oral microbial diversity and its association with ventilator-associated pneumonia in patients with mechanical ventilation
OBJECTIVE To analyze the oral microbial diversity and its association with ventilator-associated pneu-monia(VAP)in mechanically ventilated patients.METHODS Mechanically ventilated patients admitted to Guizhou Provincial People's Hospital from Oct.2022 to Oct.2023 were selected as the study subjects,and were divided in-to the non-lower respiratory infection group(Control group),the community-acquired pneumonia(CAP)group and ventilator-associated pneumonia(VAP)group,with 6 cases in each group.Oral pharyngeal specimens were sampled before oral care,followed by 16S rRNA gene sequencing to analyze their oral microorganisms.RESULTS The results of species analysis showed that the composition of dominant species of oral microbial communities was similar in CAP and VAP patients at the phylum and genus classification level.Chao1 and Shannon indices showed that the diversity and abundance of oral microorganisms in control were higher than those in CAP and VAP pa-tients(P＜0.05).There were differences in sample distribution among the control,CAP and VAP groups,with Streptococcus,Kingella,Escherichia and Acinetobacter being more abundant and varied in CAP patients,Corynebacterium,Fingoldelia and Snape being more abundant and different in VAP patients(P＜0.05),and Fusobacterium bovis and Fusobacterium haemolyticum being causive organisms in sputum cultures of CAP and VAP patients.CONCLUSION The diversity and abundance of oral microbial communities were reduced in patients with mechanical ventilation-related pneumonia,in which Acinetobacter baumannii and Acinetobacter hemolyticus infections may be associated with a high abundance of Acinetobacter in the mouth,and the analysis of oral micro-bial compositional pattern may be beneficial for VAP prediction.
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