Expression of CD36/mTORC1 pathway in peripheral blood mononuclear cells of patients with choledocholithiasis combined with infection and its diagnostic value
OBJECTIVE To investigate the expression of differentiation antigen 36(CD36)/rapamycin target com-plex 1(mTORC1)pathway in peripheral blood mononuclear cells(PBMCs)of patients with choledocholithiasis combined with infection and its diagnostic value and analyze the pathogens.METHODS A total of 79 patients with choledocholithiasis combined with infection admitted to Nantong First People's Hospital from August 2020 to Au-gust 2023 were selected and assigned as the infected group,and 83 patients without choledocholithiasis during the same period were selected as the non-infected group.The pathogenic bacteria in bile of patients with choledocholi-thiasis combined with infection and their sensitivity were analyzed.CD36/mTORC1 pathway related indexes were detected by flow cytometry and western blot,respectively.The levels of CD36,target protein of rapamycin(mTOR)and phosphorylated ribosome S6 protein kinase(p-p70S6K)in PBMCs were compared between the two groups.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of CD36,mTOR and p-p70S6K for the diagnosis of choledochal stone co-infection.RESULTS A total of 91 strains of pathogenic bacteria were cultured and isolated from patients with common bile duct stones complicated by infection,of which,62 strains of gram-negative bacteria accounted for 68.13％ ,27 strains of gram-positive bacteria accounted for 29.67％ ,and 2 strains of fungi accounted for 2.20％ ,with Escherichia coli,Klebsiella pneumoniae,and En-terococcus faecalis predominating.Escherichia coli had a high resistance rate to piperacillin(86.96％ )and ampi-cillin(82.61％ ),Klebsiella pneumoniae had a high resistance rate to ceftazidime(85.71％ )and ampicillin(76.19％ ),while both of them were sensitive to meropenem(0)and imipenem(0).Enterococcus faecalis had a high resistance rate to ampicillin(85.00％ )and clindamycin(80.00％ ),while it was sensitive to vancomycin(0)and teicoplanin(0).The levels of CD36,mTOR,and p-p70S6K in peripheral blood PBMCs of the infected group were(27.66±8.95)％ ,0.82±0.26,and 0.64±0.20,respectively,higher than those of the non-infected group(P＜0.05).The area under the curve(AUC)of the combined diagnosis of CD36,mTOR and P-P70S6K was higher than that of each single detection(P＜0.05)by plotting receiver operating characteristic(ROC)curve.CONCLUSION CD36/mTORC1 pathway was activated after choledocholithiasis co-infection,and the combined detection of CD36,mTOR,and p-p70S6K was helpful for the diagnosis of choledocholithiasis co-infection.
NETL
NSTL
万方数据
