OBJECTIVE To explore the molecular epidemiological characteristics of carbapenem resistant Klebsiella pneumoniae(CRKP)colonization in newborns.METHODS From Jan.1 to Dec.31,2021,active screening of car-bapenem resistant Enterobacteriaceae(CRE)chromogenic medium was carried out on neonatal patients admitted to 5 hospitals in Jiangxi region,and neonates identified as Klebsiella pneumoniae(KP)were included in the stud-y,and were divided into CRKP and carbapenem-sensitive K.pneumoniae(CSKP)groups according to whether car-bapenem resistant was present.The clinical characteristics(including gender,age,fever,admission diagnosis,in-flammatory indicators,antibiotic use,and whether antibiotic were used for more than 7 days)of newborns in the CRKP and CSKP groups were investigated,and the clinical characteristics of newborns with CRKP were compared with those of newborns with CSKP.At the same time,enzyme-type assay for CRKP-resistance genes was carried out to analyze the composition of different CRKP resistance genes;and the multilocus sequence typing(MLST)was performed on all CRKPs and some CSKPs.RESULTS A total of 168 CRE positive specimens were screened from 5 hospitals,and 82 KP strains were identified by mass spectrometry and paper disc method,including 25 CRKP strains and 57 CSKP strains.The average daily age of CRKP newborns was(11.64±10.86)days.Compa-ring the clinical characteristics of newborns with CRKP and CSKP,the duration of antibiotics use for more than 7 days could increase CRKP detection rate.Among the 25 CRKPs,14 strains were NDM enzymes-producing,1 strain was serine enzyme-producing,and 10 strains were non-enzymatic.In terms of the MLST typing,ST1564 and ST37 were the most common CRKPs(20.00％ of each),followed by ST2601 and ST11(16.00％ of each).CONCLUSIONS The rate of CRKP colonization was high in neonatally hospitalized children,and CRKP resistance genes were most found in metalloenzymes,ST1564,and ST37.Therefore,neonatal CRKP surveillance and infec-tion prevention and control should be strengthened.
Active screeningCarbapenem-resistant Klebsiella pneumoniaeNeonateMolecular epidemiologyMulticenter researchColonization
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