TLR2/9 gene polymorphism and drug resistance of pathogenic bacteria in neonatal bacterial meningitis
OBJECTIVE To investigate the Toll-like receptor(TLR)2/9 gene polymorphism and pathogenic bacte-ria resistance in neonatal bacterial meningitis.METHODS The clinical data of 61 newborns with bacterial meningi-tis admitted to Hainan West Central Hospital from Apr.2019 to Apr.2023 were retrospectively selected and set up as the study group,and the clinical data of 61 healthy neonates born during the same period were selected as the control group.The pathogenic bacteria and drug resistance of neonatal bacterial meningitis were analyzed.The genotype distribution of the rs5743708 locus of the TLR2 gene and the rs352140 locus of the TLR9 gene in the pe-ripheral blood of the two groups of children were detected by polymerase chain reaction(PCR-RFLP)with restric-tion fragment length polymorphism.RESULTS A total of the 64 strains of pathogenic bacteria were isolated from neonatal bacterial meningitis culture,including 38 strains of gram-negative bacteria and 26 strains of gram-positive bacteria,with Escherichia coli,Klebsiella pneumoniae and Streptococcus agalactis predominating.E.coli had a high rate of resistance to ceftriaxone(76.19％ )and cefzolin(85.71％ ),and K.pneumoniae had a high rate of re-sistance to cefzolin(100.00％ )and cefuroxime(100.00％ ),while both were more susceptible to amicacin,mero-penem and imipenem.S.agalactis had a high rate of resistance to clindamycin(100.00％ )and erythromycin(100.00％ ),while was more susceptible to ceftriaxone,meropenem and vancomycin.The frequency of AA geno-type and A allele at rs5743708 locus of the TLR2 genes in the study group were 36.07％ and 47.54％ ,respective-ly,higher than those in the control group(P＜0.05),while the frequency of G allele was 52.46％ lower than that in the control group(P＜0.05);The frequency of TT genotype and T allele at rs352140 locus of the TLR9 gene in the study group were 40.98％ and 53.28％ ,respectively,higher than those in the control group(P＜0.05),while the frequency of CC genotype and C allele frequency were 34.43％ and 46.72％ ,respectively,lower than those in the control group(P＜0.05).CONCLUSIONS Neonatal bacterial meningitis was mostly caused by gram-negative bacteria,and E.coli,K.pneumoniae and S.agalactis were the most detected pathogens,and the resistance of different pathogens to common antibiotics was different.TLR2/9 gene polymorphism may be involved in the pro-gression of neonatal bacterial meningitis.
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