Whole genome sequencing and pharmacogenomic analysis of a multidrug-resistant strain of Klebsiella pneumoniae
OBJECTIVE To sequence the whole genome of a multi-drug resistant strain of Klebsiella pneumoniae and analyze all drug resistance genes.METHODS In Oct.2023,a strain of Klebsiella pneumoniae was isolated from the urine of a patient with cerebral infarction by routine methods,identified and tested for drug sensitivity,then identified by VITEK-2 and confirmed by 16sRNA sequencing.The third-generation sequencing technology was used for whole genome sequencing.The quality control and assembly of the drug resistance genes were per-formed by bioinformatics related software,and the drug resistance function annotation platform was used to ana-lyze all resistance genes.RESULTS Klebsiella pneumoniae,No.hnyy23-147,was isolated from the urine of a male patient with cerebral infarction,and was resistant to clinically used antimicrobial drugs such as lactams,quinolones,tetracyclines,aminoglycosides,and sulfonamides,while was sensitive to polymyxin.Whole genome sequencing showed that the strain contained one chromosome and three plasmids,and all the drug resistance genes were distributed on chromosome and plasmids 2 and 3.Resistance genes included aad A2b,blasHv-159,fosA6,qnrS1,sul1,sul2,dfrA14,rmtB,blaKPC-2,blaSHv-12.Resistance caused by chromosomal mutations included acrR,ompK35,ompK36 and gyrB mutations.CONCLUSION The whole genome sequencing method can provide a timely understanding of all drug resistance genes and molecular epidemiology of multidrug-resistant Klebsiella pneumoniae,which can help to strengthen the control of nosocomial infection and the rational use of antibiotics,and is of great significance to preventing the spread of multidrug-resistant bacteria.
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