Levels of TGF-β1/Smads signaling pathway protein,Th17/Treg and pathogens from liver cancer patients with posoperative infection pulmonary
OBJECTIVE To investigate the levels of transforming growth factor-β1(TGF-β1)/Smads signaling path-way protein and helper T cell 17(Th17)regulatory T cells(Treg)in patients with postoperative pulmonary infec-tion of hepatocellular carcinoma.METHODS The clinical data of 117 patients with liver cancer who underwent liver cancer surgery in the Affiliated Hospital of Southwest Medical University from Sep.2021 to Sep.2023 were retro-spectively collected.According to the postoperative pulmonary infection,the patients were divided into the infec-tion group(n=31)and the non-infection group(n=86).The pathogenic bacteria of the patients in the infection group were analyzed,serum interleukin(IL)-6,IL-1β and TGF-β1 levels of the two groups were determined by enzyme-linked immunosorbent assay,the proportion of Th17 and Treg cells in peripheral blood was determined by flow cytometry,and the expression of Smad2,Smad3,Smad6,Smad7 and TGF-β1 protein was determined by immunoblotting.RESULTS Among 31 patients with pulmonary infection,a total of 53 strains of pathogenic bacte-ria were isolated and cultured,of which 34 strains of gram-negative bacteria,11 strains of gram-positive bacteria and 8 strains of fungi.Serum IL-6,IL-1β,TGF-β1,TGF-β1,Smad2,and Smad3 levels,the proportion of Th17 cells,and Th17/Treg in the infection group were(67.43±12.60)pg/ml,(42.56±8.71)μg/L,(301.29±54.13)pg/ml,13.26±2.05,35.21±4.67,42.89±7.91,(4.18±0.89)％and 1.69±0.28,respectively,which were higher than those in the non-infection group(P＜0.05),while the proportions of Smad6,Smad7 and Treg cells were 18.53±3.14,7.44±2.06 and(2.47±0.35)％,respectively,which were lower than those in the un-infected group(P＜0.05).CONCLUSION Postoperative pulmonary infection in patients with liver cancer mediated the activation of the TGF-β1/Smads signaling pathway by the release of inflammatory factors,and caused Th17/Treg imbalance.
Liver cancerPulmonary infectionPathogenTransforming growth factor-β1/Smads signaling path-way proteinHelper T cells 17Regulatory T cells
NETL
NSTL
万方数据
