Peripheral blood TLR4 and NF-κB levels and their clinical significance in pregnant women with threatened abortion and HCMV active infection
OBJECTIVE To investigate the levels of Toll like receptor 4(TLR4),nuclear transcription factor-κB(NF-κB)and clinical significance in peripheral blood mononuclear cells(PBMCs)of pregnant women with threat-ened abortion(TA)caused by active infection of human cytomegalovirus(HCMV).METHODS A total of 108 pregnant women with HCMV infection and TA in the Women and Children's Hospital affiliated to Ningbo Univer-sity from Mar.2021 to Mar.2023 were selected as the study group,and were divided into the active infection group(n=48)and the inactive infection group(n=60)according to the infection status,and 108 healthy preg-nant women who underwent prenatal examinations during the same period were selected as the control group.Re-ceiver operating characteristic(ROC)curves were used to analyze the diagnostic value of TLR4 and NF-κB for ac-tive HCMV infection in TA pregnant women and their predictive value for abortion outcomes.RESULTS The rela-tive expression levels of TLR4 and NF-κB mRNA in the study group(1.61±0.31)and(2.45±0.57)were high-er than those in the control group(P＜0.05);The relative expression levels of TLR4 and NF-κB mRNA in the active HCMV infection group(1.87±0.32)and(2.93±0.45)were higher than those in the inactive HCMV in-fection group(P＜0.05);The relative expression levels of TLR4 and NF-κB mRNA in the group with inevitable miscarriage caused by HCMV active infection were(2.05±0.21)and(3.27±0.42)higher than those in the group with continued pregnancy(P＜0.05).The ROC curve showed that the area under the curve(AUC)of TLR4 and NF-κB combined prediction for fetal outcomes in pregnant women with HCMV active infection was 0.906,with sensitivity and specificity of 93.75％and 71.87％,respectively.CONCLUSION The levels of TLR4 and NF-κB were elevated in pregnant women with HCMV infection and more significantly in patients with active infection,and the combination of the two could be used to predict the outcome of fetal preservation in pregnant women with HCMV active infection.
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