Predictive value of miR-34a and Wnt/β-catenin signaling pathway for postoperative infection in patients with dental implant restorations
OBJECTIVE To investigate the predictive value of miR-34a,Wnt/β-catenin signaling pathways for post-operative infection in patients with dental implant restorations and analyze the values of the indexes in prediction of the postoperative infection.METHODS A total of 211 patients who received dental implant restorations in depart-ment of stomatology,Deyang Stomatological Hospital from Oct.2021 to Sep.2023 were included in the study,and they were included in the infected group(n=60)and the non-infected group(n=151)according to whether the postoperative infection occurred.The levels of miR-34a,Wnt mRNA,β-catenin mRNA,interleukin-6(IL-6),IL-1β,and tumor necrosis factor α(TNF-α)were compared between the two groups.The correlation between miR-34a and the levels of Wnt mRNA,β-catenin mRNA,IL-6,IL-1β and TNF-α were analyzed by Pearson corre-lation analysis.Receiver operating characteristic(ROC)curve was used to analyze the predictive value of miR-34a,Wnt mRNA and β-catenin mRNA alone and in combination for postoperative infection in patients with dental im-plant restorations.RESULTS A total of 68 strains of pathogenic bacteria were co-cultured in 60 patients with post-operative infection,of which 54 were anaerobes(79.41％)and 14 were aerobes(20.59％).The miR-34a in the in-fected group was(1.58±0.46),higher than that in the non-infected group(0.98±0.35),while the levels of Wnt mRNA and β-catenin mRNA were(1.97±0.58)and(3.59±1.21),lower than those of non-infected group(2.83±0.82)and(4.67±1.69)(P＜0.05).The area under the curve(AUC)of miR-34a,Wnt mRNA and β-cate-nin mRNA alone and in combination were 0.812,0.756,0.776 and 0.891,respectively,and the predictive efficacy of the combined assay was better than that of single detection.CONCLUSION The miR-34a and Wnt/β-catenin sig-naling pathway was involved in the occurrence and development of postoperative dental implant infections,and the mechanism might be related to the targeted inhibition of the Wnt/β-catenin signaling pathway by miR-34a to pro-mote infection.
万方数据
维普
