摘要
目的 探讨牙种植修复患者术后感染微小RNA-34a(miR-34a)、Wnt/β-catenin信号通路,并分析其对术后感染的预测价值.方法 选取2021年10月-2023年9月于德阳市口腔医院口腔科接受牙种植体修复术患者211例纳入研究,根据术后是否发生感染纳入感染组(n=60)、非感染组(n=151).比较两组患者miR-34a、Wnt mRNA、β-catenin mRNA及白细胞介素-6(IL-6)、IL-1β、肿瘤坏死因子-α(TNF-α)水平.采用Pearson相关性分析法分析miR-34a与Wnt mRNA、β-catenin mRNA及IL-6、IL-1β、TNF-α的相关性.采用受试者工作特征(ROC)曲线分析miR-34a、Wnt mRNA、β-catenin mRNA对牙种植修复患者术后感染的预测价值.结果 60例术后感染患者共培养分离病原菌68株,其中厌氧菌54株(79.41％),需氧菌14株(20.59％);感染组miR-34a为(1.58±0.46)高于非感染组(0.98±0.35),Wnt mRNA、β-catenin mRNA 为(1.97±0.58)、(3.59±1.21)均低于非感染组(2.83±0.82)、(4.67±1.69)(P＜0.05);miR-34a、Wnt mRNA、β-catenin mRNA 单独及联合检测曲线下面积(AUC)分别为0.812、0.756、0.776与0.891,联合检测的预测效能优于单独检测.结论 miR-34a、Wnt/β-catenin信号通路参与牙种植体术后感染的发生与发展,其机制可能与miR-34a靶向抑制Wnt/β-catenin信号通路促进感染的发生.
Abstract
OBJECTIVE To investigate the predictive value of miR-34a,Wnt/β-catenin signaling pathways for post-operative infection in patients with dental implant restorations and analyze the values of the indexes in prediction of the postoperative infection.METHODS A total of 211 patients who received dental implant restorations in depart-ment of stomatology,Deyang Stomatological Hospital from Oct.2021 to Sep.2023 were included in the study,and they were included in the infected group(n=60)and the non-infected group(n=151)according to whether the postoperative infection occurred.The levels of miR-34a,Wnt mRNA,β-catenin mRNA,interleukin-6(IL-6),IL-1β,and tumor necrosis factor α(TNF-α)were compared between the two groups.The correlation between miR-34a and the levels of Wnt mRNA,β-catenin mRNA,IL-6,IL-1β and TNF-α were analyzed by Pearson corre-lation analysis.Receiver operating characteristic(ROC)curve was used to analyze the predictive value of miR-34a,Wnt mRNA and β-catenin mRNA alone and in combination for postoperative infection in patients with dental im-plant restorations.RESULTS A total of 68 strains of pathogenic bacteria were co-cultured in 60 patients with post-operative infection,of which 54 were anaerobes(79.41％)and 14 were aerobes(20.59％).The miR-34a in the in-fected group was(1.58±0.46),higher than that in the non-infected group(0.98±0.35),while the levels of Wnt mRNA and β-catenin mRNA were(1.97±0.58)and(3.59±1.21),lower than those of non-infected group(2.83±0.82)and(4.67±1.69)(P＜0.05).The area under the curve(AUC)of miR-34a,Wnt mRNA and β-cate-nin mRNA alone and in combination were 0.812,0.756,0.776 and 0.891,respectively,and the predictive efficacy of the combined assay was better than that of single detection.CONCLUSION The miR-34a and Wnt/β-catenin sig-naling pathway was involved in the occurrence and development of postoperative dental implant infections,and the mechanism might be related to the targeted inhibition of the Wnt/β-catenin signaling pathway by miR-34a to pro-mote infection.
维普
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