直接抗人球蛋白试验、游离抗体试验、抗体释放试验在新生儿溶血病中诊断灵敏性研究
Study on the Diagnostic Sensitivity of Direct Anti Human Globulin Test,Free Antibody Test,and Antibody Release Test in Neonatal Hemolytic Disease
林晓静1
作者信息
- 1. 361100 福建厦门,厦门市第五医院检验科
- 折叠
摘要
目的 探讨新生儿溶血病采取直接抗人球蛋白试验、游离抗体试验、抗体释放试验的诊断效果.方法 选择厦门市第五医院2020年1月1日至2023年7月17日送检的904例高胆红素血症新生儿血液样本,实施直接抗人球蛋白(抗人球蛋白组)、游离抗体(游离抗体组)及抗体释放试验(抗体释放组),比较检出结果差异并探讨诊断灵敏性.结果 阳性检出率、阴性检出率在三组中的比较,差异无统计学意义(P＞0.05).A型、B型患儿的阳性检出率在三组中比较,差异无统计学意义(P＞0.05).游离抗体组的确诊率、特异度、阳性预测高于另外两组(P＜0.05);灵敏度、阴性预测在三组中的比较,差异无统计学意义(P＞0.05);直接抗人球蛋白、游离抗体及抗体释放试验检查结果的一致性良好(Kappa=0.798,P＜0.05).结论 直接抗人球蛋白、游离抗体及抗体释放试验在新生儿溶血病中有一定应用价值,三种检查方式各有一定优劣势,临床检查诊断中可适当联合三种检查方式,以便提升新生儿溶血病临床诊断率.
Abstract
Objective To investigate the diagnostic effect of direct anti-human globulin test,free antibody test,and antibody release test for neonatal hemolytic disease.Methods Blood samples of 904 neonates with hyperbilirubinemia were selected from Xiamen Fifth Hospital from January 1,2020 to July 17,2023.Direct anti-human globulin test(anti-human globulin group),free antibody test(free antibody group),and antibody release test(antibody release group)were performed to compare the differences in detection results and explore diagnostic sensitivity.Results There was no significant difference in positive detection rate and negative detection rate among the three groups(P＞0.05).There was no significant difference in the positive detection rate of type A and type B among the three groups(P＞0.05).The diagnosis rate,specificity,and positive prediction of free antibody group were higher than those of the other two groups(P＜0.05).There was no significant difference in sensitivity and negative prediction among the three groups(P＞0.05).The results of direct anti-human globulin,free antibody,and antibody release test were consistent(Kappa=0.798,P＜0.05).Conclusion Direct antiglobulin test,free antibody test,and antibody release test are valuable in the diagnosis of hemolytic disease of newborn.In order to improve the clinical diagnosis rate of hemolytic disease of newborn,we should combine three kinds of examination methods.
关键词
贫血,溶血性,先天性/高胆红素血症,新生儿/诊断Key words
anemia,hemolytic,aAutoimmune/hyperbilirubinemia,neonatal/diagnosis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据