Objective To investigate the expression level,clinical value,biological function and potential molecular regulation mechanism of zinc transporter 4(ZIP4)in breast cancer.Methods Bioinformatics(GEPIA,UALCAN)and experimental analysis(qPCR and western blot)were used to determine the expression level of ZIP4 in the carcinoma and adjacent carcinoma of breast cancer.The UALCAN and KM plotter databases were used to further clarify the clinical and prognostic value of ZIP4 in breast cance.The biological function of ZIP4 in breast cancer was studied by cell clone formation and EDU experiments.The co-expression profile of ZIP4 in breast cancer was determined,and enrichment analysis was performed using the LinkedOmics database to clarify its underlying molecular regulatory mechanism.Results The expression of ZIP4 in breast cancer tissues was significantly higher than that in adjacent tissues.High expression of ZIP4 was associated with poor clinicopathology and prognosis in breast cancer patients.ZIP4 knockdown could significantly inhibit the proliferative ability of breast cancer cells.Furthermore,enrichment analysis indicated that ZIP4 may regulate breast cancer progression by participating in biological processes such as chromosome segregation,catalytic activity,cell cycle,and ribonucleic acid transport.Conclusion ZIP4 overexpression can significantly enhance the proliferation ability of breast cancer cells and promote the occurrence and development of breast cancer.
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