Study on the impairment of heart,liver and kidney function in rats with iron overload after repeated blood transfusion
Objective To explore the mechanism of iron overload induced by repeated blood transfusion on the function of heart,liver and kidney in SD rats.Methods The SD rat model of repeated massive blood transfusion was established,and the change of body weight and the difference of tissue mass of heart,liver and kidney were recorded,and the indexes of iron metabolism and heart,liver and kidney function in the serum of experimental animals were detected.The injury and iron deposition of heart,liver and kidney tissues were analyzed by masson and Prussian blue staining.The expression of apoptosis protein in heart,liver and kidney tissues were detected by Western blot,and the expression of apoptosis gene in heart,liver and kidney tissues were detected by Q-PCR.Results Compared with the control group,the weight and the weight of heart,liver and kidney of SD rats were significantly decreased,the level of iron ion was significantly increased,and the indexes of cardiac function such as CK and CKMB were significantly increased,the levels of Alt,AST,LDH,TBIL and DBIL were significantly increased.The histopathology staining showed that the fibrosis and iron pigment deposition in the heart,liver and kidney were obvious,the results of Western blot showed that the expression of BAX,cleaved Caspase-3 and p53 were significantly up-regulated.The results of Q-PCR showed that the expression of BAX,Caspase-3 and p53 was up-regulated,bCL-2(an inhibitor of apoptosis gene)was down-regulated.Conclusion After repeated massive blood transfusion via caudal vein in SD rats,body weight and the growth of heart,liver and kidney tissues were inhibited,serum biochemical indexes were changed,the function of heart,liver and kidney was damaged,the fibrosis of heart,liver and kidney tissues and the deposition of iron pigment were aggravated,promote the expression of apoptosis-related proteins and genes,inhibit the expression of apoptosis-inhibiting genes.
Repeated transfusionIron overloadheartLiver and kidney injuryApoptotic protein
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