细菌纤维素-明胶/丝素蛋白双层支架的制备及其性能
Preparation and properties of a bacterial cellulose gelatin/silk fibroin bilayer scaffold
孙卫华 1陈驰昊 2李喆 2吕向国 3王秀华1
作者信息
- 1. 浙江理工大学材料科学与工程学院,杭州 310018
- 2. 嘉兴学院材料与纺织工程学院,浙江嘉兴 314001
- 3. 上海交通大学医学院附属仁济医院泌尿科,上海 200127
- 折叠
摘要
为了获得能够模拟尿道组织多尺度结构的细菌纤维素复合明胶/丝素蛋白双层支架,以明胶(Gelatin,Gel)/丝素蛋白(Silk fibroin,SF)为原料,通过冷冻干燥法制备Gel/SF管状多孔支架,并以Gel/SF为模板,采用原位发酵法将细菌纤维素(Bacterial cellulose,BC)与Gel/SF支架复合,获得细菌纤维素-明胶/丝素蛋白(Gel/SF/BC)双层支架,并对Gel/SF/BC双层支架的结构及性能进行测试表征.结果表明:Gel/SF/BC双层支架具有尿道组织的宏观形貌,形成了内致密外疏松的多孔双层结构,外层以Gel/SF多孔支架为骨架,内层由单纯BC膜构成,BC纳米纤维分布在多孔孔壁表面;Gel/SF/BC双层支架具有良好的力学性能、吸水性能及生物相容性.通过该方法制备的双层支架能够高度模拟尿道组织的多尺度结构,有望应用于尿道组织的再生修复.
Abstract
To obtain a bacterial cellulose composite gelatin/silk fibroin bilayer scaffold that can simulate the multi-scale structure of urethral tissue,gelatin(Gel)/silk fibroin(SF)was used as the raw material to prepare Gel/SF tubular porous scaffold by freeze-drying method.Gel/SF was used as the template and bacterial cellulose(BC)was compounded with the Gel/SF scaffold by in situ fermentation,bacterial cellulose-gelatin/silk fibroin(Gel/SF/BC)bilayer scaffold was obtained,and the structure and performance of the Gel/SF/BC bilayer scaffold were tested and characterized.The results show that the Gel/SF/BC bilayer scaffold has a macroscopic morphology of urethral tissue,forming a porous bilayer structure with dense inner and loose outer layers.The outer layer takes the Gel/SF porous scaffold as the skeleton,and the inner layer is composed of a simple BC membrane.BC nanofibers are distributed on the surface of the porous pore wall.The Gel/SF/BC double-layer scaffold has good mechanical properties,water absorption performance,and biocompatibility.The double-layer scaffold prepared by this method can highly simulate the multi-scale structure of urethral tissue,and is expected to be applied to the regeneration and repair of urethral tissue.
关键词
双层支架/明胶/丝素蛋白/原位发酵法/细菌纤维素Key words
double layer scaffold/gelatin/silk fibroin/in situ fermentation/bacterial cellulose引用本文复制引用
基金项目
浙江省基础公益研究计划(LGG21E030010)
国家自然科学基金(51703078)
出版年
2024
NETL
NSTL
维普
万方数据