Preparation and properties of a bacterial cellulose gelatin/silk fibroin bilayer scaffold
To obtain a bacterial cellulose composite gelatin/silk fibroin bilayer scaffold that can simulate the multi-scale structure of urethral tissue,gelatin(Gel)/silk fibroin(SF)was used as the raw material to prepare Gel/SF tubular porous scaffold by freeze-drying method.Gel/SF was used as the template and bacterial cellulose(BC)was compounded with the Gel/SF scaffold by in situ fermentation,bacterial cellulose-gelatin/silk fibroin(Gel/SF/BC)bilayer scaffold was obtained,and the structure and performance of the Gel/SF/BC bilayer scaffold were tested and characterized.The results show that the Gel/SF/BC bilayer scaffold has a macroscopic morphology of urethral tissue,forming a porous bilayer structure with dense inner and loose outer layers.The outer layer takes the Gel/SF porous scaffold as the skeleton,and the inner layer is composed of a simple BC membrane.BC nanofibers are distributed on the surface of the porous pore wall.The Gel/SF/BC double-layer scaffold has good mechanical properties,water absorption performance,and biocompatibility.The double-layer scaffold prepared by this method can highly simulate the multi-scale structure of urethral tissue,and is expected to be applied to the regeneration and repair of urethral tissue.
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