Effect of Ganoderma lucidum miRNA on the senescence of human skin fibroblasts
To investigate the intervention effect and impact of miRNA in Ganoderma lucidum(G.lucidum)on cell aging,we used hydrogen peroxide(H2O2)to induce human skin fibroblasts(HSF)and constructed a cell senescence model.The HSF senescent cells were transfected with miRNA Glu-miR-01 and Glu-miR-03,which were unique to G.lucidum,and the oxidative stress indexes related to cell aging were analyzed.The change of the expression levels of age-related proteins was detected by Western blotting.The results indicated that the optimal conditions for establishing the HSF cell senescence model were 0.8 mmol/L H2O2 and 3 h induction time.Both Glu-miR-01 and Glu-miR-03 could enhance the activity of superoxide dismutase(SOD)and total antioxidant capacity(T-AOC),reduce the content of malondialdehyde(MDA),decrease the positive rate of senescence-β-galactosidase(SA-β-gal)staining and increase mitochondrial membrane potential in senescence cells.In the aging signaling pathway,the expression of P53,P21 and P16 proteins was significantly downregulated,while the expression of Rb protein was significantly upregulated.These findings suggest that Glu-miR-01 and Glu-miR-03 from G.lucidum exert protective effects on HSF senescent cells,and regulate the p53/p21/Rb signaling pathway associated with aging.This provides a theoretical basis for elucidating the molecular mechanism of G.lucidum miRNA on cell senescence.
NETL
NSTL
万方数据
维普
