High-content screening(HCS)technology combines automated high-speed imaging hardware and single-cell quantitative analysis.It can greatly accelerate data acquisition in cellular fluorescence imaging and is a powerful research technique in traditional Chinese medicine(TCM).An increasing number of laboratories and platforms,including TCM laboratories,have begun utilizing HCS systems.However,this technology is still in its infancy in TCM research and there is a lack of sufficient experience with the associated concepts,instrument configurations,and analysis methods.To improve the understanding of HCS among researchers in the field of TCM,this paper summarizes the concept of HCS,software and hardware configuration,the overall research process,as well as common problems and related solutions of HCS in TCM research based on our team's previous research experience,providing several research examples and an outlook on future perspectives,aiming to provide a technical guide for HCS in TCM research.
关键词
高内涵成像/高内涵筛选/中药
Key words
High-content imaging/High-content screening/Traditional Chinese medicine
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基金项目
Construction Fund of Key Medical Disciplines of Hangzhou(OO20200121)
Construction Fund of Key Medical Disciplines of Hangzhou(China)
"Pioneer"and"Leading Goose"R&D Program of Zhejiang(2023C03004)
"Pioneer"and"Leading Goose"R&D Program of Zhejiang(2024C03143)
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