低氧胁迫及复氧对香港牡蛎抗氧化和能量代谢相关酶活性的影响
Effects of Hypoxia-reoxygenation on Antioxidant Capacity and Enzyme Activities Related to Energy Metabolism of Crassostrea hongkongensis
张兴志 1何苹萍 1官俊良 1韦嫔媛 1江林源 1张立 1陈泳先 1罗帮 1马元 1李蔚 1李满园 1李恒慧 2彭金霞1
作者信息
- 1. 广西水产科学研究院广西水产遗传育种与健康养殖重点实验室,广西 南宁 530021
- 2. 合浦县水产技术推广站,广西 北海 536100
- 折叠
摘要
[目的]研究香港牡蛎(Crassostrea hongkongensis)对低氧的耐受性及复氧后的恢复能力,为香港牡蛎养殖及耐低氧品系选育提供参考.[方法]开展溶解氧分别为0.5、1.5、2.5 mg/L低氧胁迫72h及复氧24h的实验,分析其鳃、闭壳肌的抗氧化能力及能量代谢相关酶活性的变化情况.[结果]1)低氧胁迫6h时,0.5 mg/L及1.5 mg/L两组香港牡蛎总抗氧化能力(T-AOC)、糖原含量、丙酮酸激酶(PK)活性和磷酸果糖激酶(PFK)活性显著降低(P<0.05),磷酸烯醇式丙酮酸激酶(PEPCK)活性与对照组差异不显著(P>0.05);2)低氧胁迫72h时,T-AOC、糖原含量以及能量代谢酶PK、PFK、PEPCK活性显著升高(P<0.05);3)复氧24h后,1.5 mg/L和2.5 mg/L两个低氧组牡蛎的T-AOC,PK酶及PFK酶活性基本恢复至初始水平,与对照组相比差异不显著(P>0.05),0.5 mg/L低氧组牡蛎各项指标仍显著低于对照组,3个低氧组PEPCK酶活性均显著高于对照组(P<0.05).[结论]低氧显著影响香港牡蛎的抗氧化能力和能量代谢相关的酶活性,72 h内,牡蛎对1.5、2.5 mg/L低氧胁迫补偿适应能力较佳,但对0.5 mg/L极端低氧胁迫的补偿代谢能力较差.
Abstract
[Objective]The aim of this paper is to study the tolerance ability of Crassostrea hongkongensis to hypoxia,and provide data reference for the aquaculture and breeding of hypoxia-tolerant strain of the oysters.[Method]Experiments with 0.5,1.5,and 2.5 mg/L hypoxia stress for 72 h and reoxygenation for 24 h were carried out.The fluctuation of total antioxidant capacity(T-AOC)and enzyme activities related to energy metabolism were measured.[Result]After 6 h of hypoxia stress,the T-AOC,glycogen content,the pyruvate kinase enzyme(PK)and glycolytic phosphofructokinase enzyme(PFK)activity in 0.5 mg/L and 1.5 mg/L hypoxia groups decreased significantly(P<0.05),while the phosphoenolpyruvate carboxykinase(PEPCK)activity has no significant difference compared with the control group(P>0.05).After 72 h,the T-AOC,glycogen content and enzyme activities were increased significantly(P<0.05).After 24 hours of reoxygenation,the metabolism(T-AOC,PK and PFK activities)of oysters in 1.5 mg/L and 2.5 mg/L hypoxia groups were basically restored to normal,no significant difference compared with the control group(P>0.05),while the metabolic level of 0.5 mg/L hypoxia group was still lower than that of the control group(P<0.05).In addition,PEPCK activity in the three hypoxia groups was significantly higher than that of the control group(P<0.05).[Conclusion]Hypoxia significantly affects the antioxidant capacity and enzyme activities related to energy metabolism of Hong Kong oyster.Within 72 h,oysters had better compensatory adaptation to 1.5 and 2.5 mg/L hypoxic stress,but poor compensatory to 0.5 mg/L extreme hypoxic stress.
关键词
香港牡蛎/低氧胁迫/复氧/抗氧化能力/能量代谢Key words
Crassostrea hongkongensis/hypoxia stress/reoxygenation/antioxidant capacity/energy metabolism引用本文复制引用
基金项目
国家重点研发计划"海洋农业与淡水渔业科技创新"重点专项(2022YFD2401203)
国家重点研发计划"海洋农业与淡水渔业科技创新"重点专项(2022YFD2401205)
广西壮族自治区重点研发计划(桂科AB21196030)
广西自然科学基金(2023GXNSFBA026311)
广西水产遗传育种与健康养殖重点实验室自主研究课题(23-A-01-03)
广西水产遗传育种与健康养殖重点实验室自主研究课题(22-A-01-01)
国家贝类产业技术体系建设项目综合试验站项目(CARS-49)
现代农业产业技术体系建设项目广西创新团队项目(nycytxgxcxtd-2023-14-05)
广西农业科技自筹经费项目(Z202290)
出版年
2024
国家科技期刊平台
NSTL
万方数据