首页|低氧胁迫及复氧对香港牡蛎抗氧化和能量代谢相关酶活性的影响

低氧胁迫及复氧对香港牡蛎抗氧化和能量代谢相关酶活性的影响

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  • 国家科技期刊平台
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[目的]研究香港牡蛎(Crassostrea hongkongensis)对低氧的耐受性及复氧后的恢复能力,为香港牡蛎养殖及耐低氧品系选育提供参考.[方法]开展溶解氧分别为0.5、1.5、2.5 mg/L低氧胁迫72h及复氧24h的实验,分析其鳃、闭壳肌的抗氧化能力及能量代谢相关酶活性的变化情况.[结果]1)低氧胁迫6h时,0.5 mg/L及1.5 mg/L两组香港牡蛎总抗氧化能力(T-AOC)、糖原含量、丙酮酸激酶(PK)活性和磷酸果糖激酶(PFK)活性显著降低(P<0.05),磷酸烯醇式丙酮酸激酶(PEPCK)活性与对照组差异不显著(P>0.05);2)低氧胁迫72h时,T-AOC、糖原含量以及能量代谢酶PK、PFK、PEPCK活性显著升高(P<0.05);3)复氧24h后,1.5 mg/L和2.5 mg/L两个低氧组牡蛎的T-AOC,PK酶及PFK酶活性基本恢复至初始水平,与对照组相比差异不显著(P>0.05),0.5 mg/L低氧组牡蛎各项指标仍显著低于对照组,3个低氧组PEPCK酶活性均显著高于对照组(P<0.05).[结论]低氧显著影响香港牡蛎的抗氧化能力和能量代谢相关的酶活性,72 h内,牡蛎对1.5、2.5 mg/L低氧胁迫补偿适应能力较佳,但对0.5 mg/L极端低氧胁迫的补偿代谢能力较差.
Effects of Hypoxia-reoxygenation on Antioxidant Capacity and Enzyme Activities Related to Energy Metabolism of Crassostrea hongkongensis
[Objective]The aim of this paper is to study the tolerance ability of Crassostrea hongkongensis to hypoxia,and provide data reference for the aquaculture and breeding of hypoxia-tolerant strain of the oysters.[Method]Experiments with 0.5,1.5,and 2.5 mg/L hypoxia stress for 72 h and reoxygenation for 24 h were carried out.The fluctuation of total antioxidant capacity(T-AOC)and enzyme activities related to energy metabolism were measured.[Result]After 6 h of hypoxia stress,the T-AOC,glycogen content,the pyruvate kinase enzyme(PK)and glycolytic phosphofructokinase enzyme(PFK)activity in 0.5 mg/L and 1.5 mg/L hypoxia groups decreased significantly(P<0.05),while the phosphoenolpyruvate carboxykinase(PEPCK)activity has no significant difference compared with the control group(P>0.05).After 72 h,the T-AOC,glycogen content and enzyme activities were increased significantly(P<0.05).After 24 hours of reoxygenation,the metabolism(T-AOC,PK and PFK activities)of oysters in 1.5 mg/L and 2.5 mg/L hypoxia groups were basically restored to normal,no significant difference compared with the control group(P>0.05),while the metabolic level of 0.5 mg/L hypoxia group was still lower than that of the control group(P<0.05).In addition,PEPCK activity in the three hypoxia groups was significantly higher than that of the control group(P<0.05).[Conclusion]Hypoxia significantly affects the antioxidant capacity and enzyme activities related to energy metabolism of Hong Kong oyster.Within 72 h,oysters had better compensatory adaptation to 1.5 and 2.5 mg/L hypoxic stress,but poor compensatory to 0.5 mg/L extreme hypoxic stress.

Crassostrea hongkongensishypoxia stressreoxygenationantioxidant capacityenergy metabolism

张兴志、何苹萍、官俊良、韦嫔媛、江林源、张立、陈泳先、罗帮、马元、李蔚、李满园、李恒慧、彭金霞

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广西水产科学研究院广西水产遗传育种与健康养殖重点实验室,广西 南宁 530021

合浦县水产技术推广站,广西 北海 536100

香港牡蛎 低氧胁迫 复氧 抗氧化能力 能量代谢

国家重点研发计划"海洋农业与淡水渔业科技创新"重点专项国家重点研发计划"海洋农业与淡水渔业科技创新"重点专项广西壮族自治区重点研发计划广西自然科学基金广西水产遗传育种与健康养殖重点实验室自主研究课题广西水产遗传育种与健康养殖重点实验室自主研究课题国家贝类产业技术体系建设项目综合试验站项目现代农业产业技术体系建设项目广西创新团队项目广西农业科技自筹经费项目

2022YFD24012032022YFD2401205桂科AB211960302023GXNSFBA02631123-A-01-0322-A-01-01CARS-49nycytxgxcxtd-2023-14-05Z202290

2024

10.3969/j.issn.1673-9159.2024.02.005

广东海洋大学学报
广东海洋大学

广东海洋大学学报

CSTPCDCHSSCD北大核心
影响因子：0.444
ISSN：1673-9159
年,卷(期)：2024.44(2)
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