Identification of Marker Proteins Indicating Potential Flesh Quality Deterioration of Litopenaeus vannamei Under Low Temperature Stress and Waterless Stress
[Objective]The aim of this study is to investigate the correlation between muscle quality deterioration and differently expressed protein in hepatopancreas of Litopenaeus vannamei under low temperature(LT)stress and waterless duration(WD)stress during the anaerobic transportation,and to identify the potentially proteomic markers indicating the quality deterioration.[Method]The physiological responses of shrimp under LT stress and WD stress were observed in a simulated marine environment with a dissolved oxygen content of 7.2 mg/L and a temperature control of(12±1)℃.The significant difference of hepatopancreas proteome induced by the dual stress was analyzed by tandem mass spectrometry,and its correlation with muscle quality was analyzed accordingly.[Results]Compared with the control,33 and 44 differently expressed proteins were identified in LT and LT+WD groups,respectively,among which the most DEPs were significantly enriched in lysosomal,glycolytic or gluconeogenic,and adhesion pathways(P<0.05),while trypsin-1 and dipeptidyl peptidase underwent significant up-regulatied(P<0.05)and phosphoglycan isomerase and aldehyde dehydrogenase were significantly down-regulated(P<0.05).Moreover,the deterioration in colour and texture attributes was closely associated with the expression of tubulin,gelsolin,laminin,endogenous protease and metabolic enzymes.[Conclusion]This research found out that the intrinsic relationship between hepatopancreas DEPs and muscle quality for the first time.The identified typical DEPs could be used as potential marker proteins indicating quality deterioration,and thus providing a molecular target for monitoring muscle quality deterioration of L.vannamei during waterless live transport.
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