Review on Application of Single-cell Transcriptome Sequencing Technology in Aquaculture
Single-cell RNA sequencing(scRNA-seq)can reveal the heterogeneity between cells in different tissues as well as various cells in the same tissue.At present,it has been frequently used in model species,but the application in non-model species is relatively less reported,with particularly sparse reports in aquaculture species.We review the application of scRNA-seq technology in artificial reproduction,breeding,disease prevention,and control of aquaculture species.We also point out the difficulties in sample collection,single cell separation and data analysis in the application process,and propose corresponding solutions,and look forward to the development prospects of this technology within the aquaculture field.It provides a reference for promoting the widespread application of scRNA-seq technology in aquaculture species.