Effects of Extracting Strategies on the Yield,Conformation,and Thermostability of Myofibrillar Proteins in Shrimp Flesh
[Objective]The aim of this study is to obtain high-quality myofibrillar proteins in shrimp(Litopenaeus vannamei).[Methods]The study evaluated the effects of four different extraction methods on the spatial structure of myofibrillar proteins.The 4 methods are(1)53 kHz ultrasound-assisted 0.5 mol/L KCl,(2)0.5 mol/L KCl,(3)53 kHz ultrasound-assisted 5%sodium dodecyl sulphate(SDS),and(4)RIPA lysate.The evaluation indexes used were spectroscopy and biochemical analyses of gel electrophoresis to determine the myofibrillar protein extraction rate and structural integrity.[Results]The extraction rates by the four methods were(87.99±3.08)%,(53.76±3.9)%,(75.59±0.95)%,and(95.46±4.49)%,respectively,when using.The higher protein abundance in troponin and tropomyosin was observed when the proteins were extracted by the method(1).The third and the second methods produced the higher abundance in myosin heavy chain(MHC)and actin proteins.Overall,the ultrasound-assisted extraction maintains the natural structure of myofibrillar protein to the maximum extent,the second method maintains with the characteristics of the high levels of α-helix content and intact amide I band structure.However,RIPA extraction damages the secondary structure of myofibrillar protein,by which the thermal stability reduced to(60.63±4.46)%,as compared to its original proteins.[Conclusion]The comprehensive results from extraction yield,spatial structure analysis,and thermal stability tests indicate that the third method yields the optimal extraction quality of myofibrils from shrimp.This study obtains the optimal extracting strategy for high quality myofibrillar protein in shrimp flesh,and provides the basis for elucidating the underlying mechanism and developing effective method against quality deterioration during refrigeration.
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