Isolation and Identification of Dominant Pseudomonas spp.from Tilapia under Super-chilled Storage and Ice-tolerant Genes of Pseudomonas Strains
[Objective]The aim of this study is to analyze the unique genetic characteristics and ice-tolerant genes of dominant ice-tolerant Pseudomonas strains isolated from tilapia.[Methods]The dominant Pseudomonas strains were first identified using full-length 16S rDNA sequencing from tilapia under super-chilled storage.These strains were then isolated,identified,and subjected to de novo genome sequencing using the Illumina platform.Gene family analysis was performed to determine the core gene families of these ice-tolerant Pseudomonas strains,which were subsequently compared to the genome of the ice-intolerant Pseudomonas aeruginosa PAO1 to identify the unique core gene families of these and then screened out the potential ice-tolerant genes in combination with the cold-adaptive genes of Pseudomonas reported in the literature.[Result and conclusion]Five strains of spoilage bacteria with the highest abundance in tilapia under super-chilled storage were discovered and isolated,and they were identified as Pseudomonas bubulae and Pseudomonas fragi.These five Pseudomonas strains shared 3 692 core gene families,of which 861 were absent in the genome of ice-intolerant P.aeruginosa.These 861 unique core gene families were significantly enriched in pathways related to quorum sensing,steroid degradation,metabolism of certain amino acids and peptides,fatty acid degradation,ascorbate and aldarate metabolism,limonene and pinene degradation,butanoate metabolism,styrene degradation,geraniol degradation and others.Further screening revealed that these five ice-tolerant Pseudomonas strains possessed the potential ice-tolerant genes,such as des,cbpM,ousA,treP,treR,trxC,and grX,which were absent in the genome of ice-intolerant P.aeruginosa.
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