Isolation of Tetragenococcus halophilus from Different Sources and Correlation Between Their Salt and Acid Tolerance and Gene Features
[Objective]To compare the differences in the core gene families of better and worse strains of Tetragenococcus halophilus in terms of salt and acid tolerance and screen the metabolic pathways and genes that may be related to salt and acid tolerance.[Methods]T.halophilus were isolated from highly salt aquatic products and soy sauce mash,identified by 16S rDNA sequencing.Their physiological and biochemical features were identified and salt and acid tolerance were tested.The strains with obvious differences in salt and acid resistance were classified respectively.Further genome sequencing was performed on 17 strains based on Illumina platform to compare the differences in the core gene families of the strains with obvious differences in salt and acid resistance.Respectively,the KEGG pathway and GO function were enriched and analysed for the specific core gene families of the strains with better salt-and acid-tolerance abilities.[Results and conclusion]All 17 strains differed in physiological and biochemical characterization,but all showed negativity for β-galactoside and xylose,and positivity for fructose,sorbose and D-ribose.Tolerance results showed significant differences among the strains.The results of genome sequencing indicated that the strains with better salt tolerance possessed 294 unique core gene families,which were mainly enriched in the KEGG pathways of pentose phosphate pathway,methane metabolism,metabolism of ascorbic acid and aldic acid,and phosphotransferase system (PTS),whilein GO function,were mainly enriched in the processes of localization,transport,establishment of localization,DNA recombination,and DNA metabolism.The strains with better acid tolerance had 191 unique core gene families,which were mainly enriched in the KEGG pathways of fructose and mannose metabolism,starch and sucrose metabolism,carotenoid biosynthesis,amino acid and nucleotide metabolism,and PTS,while in GO function,were mainly enriched in DNA recombination and DNA metabolism processes.The above pathways and functions may encompass gene features associated with salt and acid resistant properties of T.halophilus.Further analysis revealed that the genes enriched in the PTS were exactly the same for the two unique core gene families,and these genes might beclosely related to the resistance mechanism of T.halophilus.
万方数据
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