The biological indicators of cultured Sepiella maindroni collected in Xixuan island farm in Zhoushan city were analyzed to explore the reproductive mode of the male cultured ones.The effective fecun-dity of male individuals and its variations with biological indicators were assessed using model fitting analysis.The specimens were measured 32-102 mm in mantle length and weighted from 5.3-128.7 g.The Gonadosomat-ic index ranging from 1.87%to 3.64%and Spermatophoric complex index ranging from 0.32%to 4.00%,re-spectively,and both increased gradually with gonad development.The length and weight of spermatophoric sac increased with sexual maturation measured 25-55 mm in length and 0.30-1.54 g in weight,respectively.The spermatophoric sac's weight showed a linear regression on its length,andbothof them reached to the highest value at the stage Ⅵ.The effective fecundity was estimated from 41 to 3 824 spermatophores and the relative effective fecundity by mantle length was estimated from 0.52 to 56.24 spermatophores per millimeter,which in-creased gradually with the growth and development of individuals and reached the maximum at stage Ⅵ.The spermatophores are measured from 1.74 to 12.87 mm in length and from 0.000 1 to 0.002 6 g in weight,re-spectively.