Genome-Wide Survey Analysis and Microsatellite Distribution Features of Setipinna tenuifilis
In this study,the whole genome of Setipinna tenuifilis was sequenced,and genome-wide survey analysis and microsatellite feature studies were conducted to evaluate its basic information and provide impor-tant information for further research on the species'genetic diversity and evolution.The study showed that S.tenuifilis genome size is approximately 815 Mb,with a repeat sequence proportion of 39.69％and a heterozy-gosity rate of 1.77％.With the preliminary assembly,the contig N50 was 35 836 bp and the scaffold N50 was 107 605 bp.In addition,14 562 379 microsatellite loci were detected in the whole genome,with a relative abundance of approximately 1 713 microsatellite motifs per Mb.Among them,the dinucleotide repeat was the most common(90.64％),followed by the mononucleotide repeat(5.52％),and the hexanucleotide repeat was less frequent(0.24％).The microsatellite repeat copy number was mainly distributed between 6 and 15 times,and AC/GT and AG/CT were the most common dinucleotide repeat types.These research results provide important in-formation for genetic and evolutionary biology studies of S.tenuifilis and useful molecular markers for the pro-tection of its genetic diversity.
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